Expression of the mevalonate pathway enzymes in the Lutzomyia longipalpis (Diptera: Psychodidae) sex pheromone gland demonstrated by an integrated proteomic approach

dc.creatorGonzález-Caballero, Natalia
dc.creatorRodríguez-Vega, Andrés
dc.creatorDias-Lopes, G.
dc.creatorValenzuela, J. G.
dc.creatorRibeiro, J. M.
dc.creatorCarvalho, Paulo Costa
dc.creatorValente, Richard Hemmi
dc.creatorBrazil, R. P.
dc.creatorCuervo, P.
dc.date2016-09-05T14:38:28Z
dc.date2016-09-05T14:38:28Z
dc.date2014
dc.date.accessioned2023-09-26T20:37:16Z
dc.date.available2023-09-26T20:37:16Z
dc.identifierGONZÁLEZ-CABALLERO, N. et al. Expression of the mevalonate pathway enzymes in the Lutzomyia longipalpis (Diptera: Psychodidae) sex pheromone gland demonstrated by an integrated proteomic approach. Journal of Proteomics, n. 96, p. 117-132, Jan. 2014.
dc.identifier1874-3919
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/15606
dc.identifier10.1016/j.jprot.2013.10.028
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8860732
dc.descriptionThis work was funded by the Coordenação de Aperfeiçoamento de Pessoal de Nivel Superior (CAPES), Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ) and Programa de Desenvolvimento Tecnológico em Insumos para Saúde (PDTIS-Fiocruz).
dc.descriptionIn Latin America, Lutzomyia longipalpis is the main vector of the protozoan parasite Leishmania infantum, which is the causal agent of American Visceral Leishmaniasis. This insect uses male-produced pheromones for mate recognition. Elucidation of pheromone biogenesis or its regulation may enable molecular strategies for mating disruption and, consequently, the vector's population management. Motivated by our recent results of the transcriptomic characterization of the L. longipalpis pheromone gland, we performed a proteomic analysis of this tissue combining SDS-PAGE, and mass spectrometry followed by an integrative data analysis. Considering that annotated genome sequences of this sand fly are not available, we designed an alternative workflow searching MS/MS data against two customized databases using three search engines: Mascot, OMSSA and ProLuCID. A total of 542 proteins were confidently characterized, 445 of them using a Uniref100-insect protein database, and 97 using a transcript translated database. In addition, use of PEAKS for de novo peptide sequencing of MS/MS data confirmed ~90% identifications made with the combination of the three search engines. Our results include the identification of six of the seven enzymes of the mevalonate-pathway, plus the enzymes involved in sesquiterpenoid biosynthesis, all of which are proposed to be involved in pheromone production in L. longipalpis.
dc.formatapplication/pdf
dc.languagepor
dc.rightsopen access
dc.subjectLutzomyia longipalpis
dc.subjectMale pheromone gland
dc.subjectMevalonate pathway
dc.subjectProteome
dc.subjectProteomics
dc.titleExpression of the mevalonate pathway enzymes in the Lutzomyia longipalpis (Diptera: Psychodidae) sex pheromone gland demonstrated by an integrated proteomic approach
dc.typeArticle


Este ítem pertenece a la siguiente institución