A new Aura virus isolate in Brazil shows segment duplication in the variable region of the nsP3 gene

dc.creatorMosimann, Ana Luiza Pamplona
dc.creatorSiqueira, Mirian Krystel de
dc.creatorCeole, Lígia Fernanda
dc.creatorSantos, Claudia Nunes Duarte dos
dc.date2018-11-14T12:45:44Z
dc.date2018-11-14T12:45:44Z
dc.date2018
dc.date.accessioned2023-09-26T20:36:40Z
dc.date.available2023-09-26T20:36:40Z
dc.identifierMOSIMANN, Ana Luiza Pamplona et al. A new Aura virus isolate in Brazil shows segment duplication in the variable region of the nsP3 gene. Parasites & Vectors, v. 11, n. 321, p. 1-10, 2018.
dc.identifier1756-3305
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/30022
dc.identifier10.1186/s13071-018-2907-4
dc.identifier1756-3305
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8860548
dc.descriptionA new isolate of Aura virus serendipitously discovered as a cell culture contaminant is reported in this manuscript. Aura virus belongs to the family Togaviridae and is classified in the genus Alphavirus. There are only two reports of Aura virus isolation from mosquitoes in the scientific literature, and the existence of a vertebrate host is still unknown. The discovery of this new isolate was based on transmission electron microscopy and nucleic acid amplification through a non-specific RT-PCR amplification protocol followed by sequencing. Then, genetic analysis has shown that the new virus shares a high degree of identity with the previously described isolate (GenBank: AF126284.1). A major difference was observed in the nsP3 gene in which a 234-nucleotide duplication has been identified. Furthermore, a pronounced difference was observed in cell cultures compared to the data available for the previously described isolate. Cell permissiveness and phenotypic characteristics in C6/36, Vero and BHK-21 cells were found to differ from previous reports. This may be due to the genetic differences that have been observed.This way, concluded the genetic and biological characteristics of the new Aura virus isolate are suggestive of viral adaptation to the cell substrate. The development of a cDNA clone will lend a perspective and better understanding of these results as well as open avenues for its use as a biotechnological tool, as seen for other alphaviruses.
dc.formatapplication/pdf
dc.languagepor
dc.publisherBMC
dc.rightsopen access
dc.subjectAura virus
dc.subjectTogaviridae Infections
dc.subjectGene Duplication
dc.subjectInfecciones por Togaviridae
dc.subjectDuplicación de Gen
dc.subjectAlphavirus
dc.subjectInfecções por Togaviridae
dc.subjectDuplicação Gênica
dc.titleA new Aura virus isolate in Brazil shows segment duplication in the variable region of the nsP3 gene
dc.typeArticle


Este ítem pertenece a la siguiente institución