A new repetitive DNA sequence from Trypanosoma cruzi

dc.creatorLima, Leila de Mendonça
dc.creatorTraub-Cseko, Yara M.
dc.date2020-09-18T19:37:01Z
dc.date2020-09-18T19:37:01Z
dc.date1991
dc.date.accessioned2023-09-26T20:36:36Z
dc.date.available2023-09-26T20:36:36Z
dc.identifierLIMA, Leila de Mendonça; TRAUB-CSEKO, Yara M. A new repetitive DNA sequence from Trypanosoma cruzi. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 86, n. 4, p. 475, Oct./Dec. 1991.
dc.identifier0074-0276
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/43464
dc.identifier10.1590/S0074-02761991000400020
dc.identifier1678-8060
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8860524
dc.descriptionTandemly repeated DNA sequences are found in the genome of higher eukaryotes, and have also been demonstrated in Trypanosoma cruzi. Repeated DNA sequences are potentially useful for the diagnostic detection of T. cruzi (A. Gonzales et al., 1984, Proc. Natl. Acad. Sci. USA, 81: 3356-3360). We have isoleted two clones from a genomic library of T. cruzi (Y strain) that contain, in one clone a family of at least seven copies of a repetitive sequence of approximately 600 base pairs, and in the other an independent copy of the same sequence. One copy of the repetition (HSP) and the independent clone (HCR) were sequenced by the Sanger procedure (Fig.). This sequence hybridized to four strains of T. cruzi tested and did not hybridize to eleven species of trypanosotids from five different Genera, being a good candidate for diagnostic assays.
dc.formatapplication/pdf
dc.languageeng
dc.publisherFundação Oswaldo Cruz. Instituto Oswaldo Cruz.
dc.rightsopen access
dc.subjectTrypanosoma cruzi
dc.subjectSequência de DNA
dc.subjectDNA sequence
dc.subjectTrypanosoma cruzi
dc.titleA new repetitive DNA sequence from Trypanosoma cruzi
dc.typeArticle


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