The purinergic receptor P2X7 role in control of Dengue virus-2 infection and cytokine/chemokine production in infected human monocytes

dc.creatorCorrêa, Gladys
dc.creatorLindenberg, Carolina de A.
dc.creatorSantos, Caroline Fernandes
dc.creatorGandini, Mariana
dc.creatorPaiva, Fabienne Petitinga
dc.creatorSilva, Robson Coutinho
dc.creatorKubelka, Claire F.
dc.date2017-03-23T13:45:58Z
dc.date2017-03-23T13:45:58Z
dc.date2016
dc.date.accessioned2023-09-26T20:36:17Z
dc.date.available2023-09-26T20:36:17Z
dc.identifierCORRÊA, Gladys; et al. The purinergic receptor P2X7 role in control of Dengue virus-2 infection and cytokine/chemokine production in infected human monocytes. Immunology, v.221, n.7, p.794-802, July 2016.
dc.identifier0171-2985
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/18131
dc.identifier10.1016/j.imbio.2016.02.003
dc.identifier1878-3279
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8860429
dc.descriptionPurinergic signaling has a crucial role in intracellular pathogen elimination. The P2X7 purinergic receptor (P2X7R), once activated by ATP, leads to pro-inflammatory responses including reactive oxygen species production. ATP can be released by injured cells, as endogenous danger signals. Dengue fever may evolve to a severe disease, leading to hypovolemic shock and coagulation dysfunctions as a result of a cytokine storm. Our aim was to evaluate the role of P2X7R activation during Dengue virus (DENV) infection. Extracellular ATP inhibited viral load in pretreated monocytes, as measured by NS1 secretion and by decrease in DENV(+) P2X7(+) cell frequencies, suggesting that P2X7R is involved in the antiviral response. Nitric oxide (NO) has anti-DENV properties and is decreased after DENV infection. NO production after ATP stimulation is abrogated by KN62 treatment, a specific P2X7R inhibitor, indicating that P2X7R likely is acting in the virus containment process. Additionally, TNF, CXCL8, CCL2 and CXCL10 factors that are associated with dengue severity were modulated by the P2X7R activation. We conclude that P2X7R is directly involved in the modulation of the antiviral and inflammatory process that occurs during DENV infection in vitro, and may have an important role in patient recovery in a first moment.
dc.description2030-01-01
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsrestricted access
dc.subjectReceptor P2X7
dc.subjectDengue
dc.subjectMonócitos
dc.subjectSinalização purinérgica
dc.subjectP2X7 receptor
dc.subjectDengue
dc.subjectMomocytes
dc.subjectPurinergic signaling
dc.titleThe purinergic receptor P2X7 role in control of Dengue virus-2 infection and cytokine/chemokine production in infected human monocytes
dc.typeArticle


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