Lectin-induced nitric oxide production

dc.creatorAndrade, Jó Luis
dc.creatorArruda, Sérgio Marcos
dc.creatorBessa, Theolis Costa Barbosa
dc.creatorPaim, Luciana
dc.creatorRamos, Márcio Viana
dc.creatorCavada, Benildo Sousa
dc.creatorBarral Netto, Manoel
dc.date2012-10-05T17:40:00Z
dc.date2012-10-05T17:40:00Z
dc.date1999
dc.date.accessioned2023-09-26T20:35:55Z
dc.date.available2023-09-26T20:35:55Z
dc.identifierANDRADE, J.L. et al. Lectin-induced nitric oxide production. Cellular Immunology, v. 194, n. 1, p. 98-102, may.1999.
dc.identifier0008-8749
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/5650
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8860309
dc.descriptionConsidering that nitric oxide (NO) may be involved in anti-tumoral and anti-parasite lectin effects, in this report we investigated whether lectin induces NO production. Lectins from Canavalia brasiliensis, Dioclea grandiflora, Pisum arvense (PAA), and concanavalin A induced murine peritoneal cells to produce NO in vitro. PAA induced similar levels to that obtained with lipopolysaccharide plus interferon-gamma. NO production by adherent cells was significantly lower than that of unfractionated cells, suggesting a combination of lectin stimuli directly on macrophages and via lymphocyte stimulation. Ex vivo experiments showed that cells stimulated in vivo could maintain NO production in vitro without further stimuli. NO synthesis blockage in vivo can significantly increase cell numbers in draining lymph nodes after lectin injection compared to unblocked controls, suggesting an in vivo association of lectin stimuli and NO production. Taken together these data show that lectins can induce NO production both in vitro and in vivo.
dc.formatapplication/pdf
dc.languageeng
dc.rightsopen access
dc.subjectNitric Oxide
dc.subjectAdherent cells
dc.subjectPeritoneal cells
dc.subjectMacrophages
dc.subjectImmunology
dc.subjectCanavalia Brasiliensis
dc.subjectCanavalia Ensiformis
dc.subjectConcanavalin A
dc.subjectConA
dc.subjectDioclea Grandiflora
dc.subjectPisum Arvense
dc.subjectLectinas/imunologia
dc.subjectÓxido Nítrico/biossíntese
dc.subjectLectinas de Plantas
dc.subjectAnimais
dc.subjectCélulas Cultivadas
dc.subjectConcanavalina A/imunologia
dc.subjectConcanavalina A/farmacologia
dc.subjectFeminino
dc.subjectLectinas/farmacologia
dc.subjectCamundongos
dc.subjectCamundongos Endogâmicos BALB C
dc.subjectPeritônio/citologia
dc.titleLectin-induced nitric oxide production
dc.typeArticle


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