Antiserum against perimicrovillar membranes and midgut tissue reduces the development of Trypanosoma cruzi in the insect vector, Rhodnius prolixus

dc.creatorGonzalez, M. S.
dc.creatorHamedi, A.
dc.creatorCunha, J. M. Albuquerque
dc.creatorNogueira, N. F. S.
dc.creatorSouza, W. de
dc.creatorRatcliffe, N. A.
dc.creatorAzambuja, P.
dc.creatorGarcia, E. S.
dc.creatorMello, C. B.
dc.date2020-01-21T12:03:08Z
dc.date2020-01-21T12:03:08Z
dc.date2006
dc.date.accessioned2023-09-26T20:35:36Z
dc.date.available2023-09-26T20:35:36Z
dc.identifierGONZALEZ, M. S. et al. Antiserum against perimicrovillar membranes and midgut tissue reduces the development of Trypanosoma cruzi in the insect vector, Rhodnius prolixus. Experimental Parasitology, v. 114, n. 4, p. 297-304, Dec. 2006.
dc.identifier0014-4894
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/39335
dc.identifier10.1016/j.exppara.2006.04.009
dc.identifier1090-2449
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8860204
dc.descriptionAntiserum raised against Rhodnius prolixus perimicrovillar membranes (PMM) and midgut tissue interfered with the midgut structural organization and reduced the development of Trypanosoma cruzi in the R. prolixus insect vector. SDS-PAGE and Western blot analyses confirmed the specific recognition of midgut proteins by the antibody. Feeding, mortality, molt, and oviposition of the insects were unaffected by feeding with the antiserum. However, the eclosion of the eggs were reduced from R. prolixus females treated with antiserum. Additionally, in vivo evaluation showed that after oral treatment with the antiserum, the intensity of infection with the Dm-28c clone of T. cruzi decreased in the digestive tract of fifth-instar nymphs and in the excretions of R. prolixus adults. These results suggest that the changes observed in the PMM organization in the posterior midgut of R. prolixus may not be important for triatomine survival but the antiserum acts as a transmission-reduction vaccine able to induce significant decreases in T. cruzi infection in the vector.
dc.description2022-01-01
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsrestricted access
dc.subjectTrypanosoma cruzi
dc.subjectAnti-soro
dc.subjectMembranas peritróficas e perimicrovilares
dc.subjectProtozoários
dc.subjectTrypanosoma cruzi (protozoan)
dc.subjectRhodnius prolixus
dc.subjectPeritrophic and perimicrovillar membranes
dc.subjectAntiserum
dc.titleAntiserum against perimicrovillar membranes and midgut tissue reduces the development of Trypanosoma cruzi in the insect vector, Rhodnius prolixus
dc.typeArticle


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