An improved method to obtain antigen-excreting Toxocara canis larvae.

dc.creatorNeves, Neuza Maria Alcântara
dc.creatorSantos, Alex Barbosa dos
dc.creatorMendonça, Lívia Ribeiro
dc.creatorFigueiredo, Camila Alexandrina Viana
dc.creatorPontes-de-Carvalho, Lain Carlos
dc.date2014-09-02T11:41:25Z
dc.date2014-09-02T11:41:25Z
dc.date2008
dc.date.accessioned2023-09-26T20:35:04Z
dc.date.available2023-09-26T20:35:04Z
dc.identifierALCÂNTARA-NEVES, N. M. et al. An improved method to obtain antigen-excreting Toxocara canis larvae. Experimental Parasitology, v. 119, n. 3, p. 349-351, 2008.
dc.identifier1090-2449
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/8300
dc.identifier10.1016/j.exppara.2008.03.006
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8860025
dc.descriptionToxocara canis is a dog helminth which causes visceral larva migrans (VLM) when infecting humans as a larva. The infection is demonstrated by detecting IgG antibodies against excretory–secretory larval antigens (ESLA) in serum by ELISA. The production of ESLA involves the collection of adult worms from dog puppy stools, the separation of eggs from dissected uteri, and the in vitro growing of egg-derived larvae, following the time-consuming and laborious protocol described by De Savigny [De Savigny, D.H., 1975. In vitro maintenance of T. canis larvae and a simple method for the production of Toxocara ES antigen for the uses in serodiagnostic tests for visceral larva migrans. Journal of Parasitology 61, 781–782]. In this work, an improved protocol for obtaining T. canis larvae is described. The modifications proposed improved the efficiency of the original De Savigny method in three ways: (i) increasing the parasite yield up to five fold, (ii) improving the larval purity, and (iii) markedly reducing the execution time of the protocol.
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier Inc
dc.rightsopen access
dc.subjectESLA
dc.subjectExcretory–secretory larval antigens
dc.subjectELISA
dc.subjectEnzyme-linked immunosorbent assay
dc.subjectToxocara canis
dc.subjectLarva purification
dc.subjectDoenças do Cão/parasitologia
dc.subjectToxocara canis/isolamento & purificação
dc.subjectToxocaríase/parasitologia
dc.subjectAnimais
dc.subjectAntígenos de Helmintos/metabolismo
dc.subjectCães
dc.subjectFezes/parasitologia
dc.subjectFeminino
dc.subjectHumanos
dc.subjectLarva Migrans Visceral/diagnóstico
dc.subjectToxocara canis/imunologia
dc.titleAn improved method to obtain antigen-excreting Toxocara canis larvae.
dc.typeArticle


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