Neutrophil extracellular traps release induced by Leishmania: role of PI3Kγ, ERK, PI3Kσ, PKC, and [Ca2+]

dc.creatorVieira, Thiago de Souza
dc.creatorCosta, Anderson Guimarães
dc.creatorRochael, Natalia C.
dc.creatorLira, Maria N.
dc.creatorNascimento, Michelle T.
dc.creatorGomez, Phillipe de Souza Lima
dc.creatorMariante, Rafael M.
dc.creatorPersechini, Pedro M.
dc.creatorSaraiva, Elvira M.
dc.date2017-04-18T17:59:10Z
dc.date2017-04-18T17:59:10Z
dc.date2016
dc.date.accessioned2023-09-26T20:33:45Z
dc.date.available2023-09-26T20:33:45Z
dc.identifierVIEIRA, Thiago de Souza; et al. Neutrophil extracellular traps release induced by Leishmania: role of PI3Kg, ERK, PI3Ks, PKC, and [Ca2+]. Journal of Leukocyte Biology, v.100, 9p, nov. 2016.
dc.identifier0741-5400
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/18509
dc.identifier10.1189/jlb.4A0615-261RR
dc.identifier1938-3673
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8859578
dc.descriptionUpon in vitro stimulation, neutrophils undergo a cell death named netosis. This process is characterized by extracellular release of chromatin scaffold associated with granular and cytoplasmic proteins, which together, ensnare and kill microbes. We have previously described that interaction of Leishmania amazonensis with human neutrophils leads to the release of neutrophil extracellular traps, which trap and kill the parasite. However, the signaling leading to Leishmania induced netosis is still unknown. Thus, we sought to evaluate signaling events that drive L. amazonensis induced neutrophil extracellular trap release from human neutrophils. Here, we found that PI3K, independently of protein kinase B, has a role in parasite-induced netosis. We also described that the main isoforms involved are PI3Kγ and PI3Kδ, which work in reactive oxygen species-dependent and -independent ways, respectively. We demonstrated that activation of ERK downstream of PI3Kγ is important to trigger reactive oxygen species-dependent, parasite-induced netosis. Pharmacological inhibition of protein kinase C also significantly decreased parasite-induced neutrophil extracellular trap release. Intracellular calcium, regulated by PI3Kδ, represents an alternative reactive oxygen species-independent pathway of netosis stimulated by L. amazonensis Finally, intracellular calcium mobilization and reactive oxygen species generation are the major regulators of parasite-induced netosis. Our results contribute to a better understanding of the signaling behind netosis induced by interactions between Leishmania and neutrophils.
dc.description2030-01-01
dc.formatapplication/pdf
dc.languageeng
dc.publisherSociety for Leukocyte Biology
dc.rightsrestricted access
dc.subjectLeishmania
dc.subjectNeutrófilos
dc.subjectLiberação de armadilhas extra-celulares
dc.subjectLeishmania
dc.subjectNeutrophil
dc.subjectextracellular traps release
dc.titleNeutrophil extracellular traps release induced by Leishmania: role of PI3Kγ, ERK, PI3Kσ, PKC, and [Ca2+]
dc.typeArticle


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