Molecular characterization of uropathogenic and diarrheagenic Escherichia coli pathotypes

dc.creatorMangia, Adriana Hamond Regua
dc.creatorIrino, Kinue
dc.creatorPacheco, Raquel da Silva
dc.creatorBezerra, Rose Mary Pimentel
dc.creatorPérissé, André Reynaldo Santos
dc.creatorTeixeira, Lucia Martins
dc.date2018-04-10T15:38:42Z
dc.date2018-04-10T15:38:42Z
dc.date2010
dc.date.accessioned2023-09-26T20:32:57Z
dc.date.available2023-09-26T20:32:57Z
dc.identifierREGUA-MANGIA, Adriana Hamond et al. Molecular characterization of uropathogenic and diarrheagenic Escherichia coli pathotypes. Journal of Basic Microbiology, v. 50, Supl. 1, p. S107-S115, 2010.
dc.identifier0233-111X
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/25730
dc.identifier10.1002/jobm.200900364
dc.identifier1521-402
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8859295
dc.descriptionIn this study diarrheagenic and uropathogenic Escherichia coli (UPEC) strains were comparatively characterized according to serotype, hemolytic activity, protein polymorphism among housekeeping enzymes, phylogenetic group and urovirulence genes. Intra-serogroup/serotype variations were observed. Hemolytic activity was detected in 100%, 93%, 67% and 39% of UPEC, EAEC, EPEC and ETEC strains, respectively. The alpha-hemolytic phenotype was observed in all pathogenic groups while beta-hemolytic phenotype was less frequent. PCR phylotyping revealed higher prevalence of diarrheagenic E. coli in groups A and D while uropathogenic strains were mainly found in subgroup B2. Amplification assays revealed that 74%, 45% and 22% of UPEC, EAEC and EPEC strains, respectively, carried at least one of the urovirulence sequences. The molecular typing system revealed a pathotype-specific clonal group distribution and showed a closer relationship between the EAEC and UPEC. Additionally, the occurrence of urovirulence traits, especially those related to iron acquisition, was more frequent among EAEC and UPEC than among the other E. coli pathotypes. This observation is of special value considering that the EAEC pathotype constitutes an emerging group of enteropathogens, particularly, in developing countries, and information on their pathogenic and phylogenetic characteristics is still scarce.
dc.description2030-01-01
dc.formatapplication/pdf
dc.languageeng
dc.publisherWiley-VCH Verlag
dc.rightsrestricted access
dc.subjectCaracterização Molecular
dc.subjectEscherichia coli
dc.subjectVirulência
dc.subjectPatótipos
dc.subjectEscherichia coli pathotypes
dc.subjectMolecular characterization
dc.subjectVirulence
dc.titleMolecular characterization of uropathogenic and diarrheagenic Escherichia coli pathotypes
dc.typeArticle


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