Identification of new repetitive element in Leptospira interrogans serovar copenhageni and its application to PCR-based differentiation of Leptospira serogroups.

dc.creatorBarocchi, Michele A
dc.creatorKo, Albert Icksang
dc.creatorFerrer, Suzana Ramos
dc.creatorFaria, Marcos Tucunduva de
dc.creatorReis, Mitermayer Galvão dos
dc.creatorRiley, Lee Woodland
dc.date2014-05-02T14:12:01Z
dc.date2014-05-02T14:12:01Z
dc.date2001
dc.date.accessioned2023-09-26T20:32:14Z
dc.date.available2023-09-26T20:32:14Z
dc.identifierBAROCCHI, M. A. et al. Identification of new repetitive element in Leptospira interrogans serovar copenhageni and its application to PCR-based differentiation of Leptospira serogroups.
dc.identifier0095-1137
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/7567
dc.identifier10.1128/JCM.39.1.191–195.2001
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8859061
dc.descriptionA new repetitive DNA element was identified in an isolate of Leptospira interrogans serovar copenhageni from a patient in Salvador, Brazil. A Sau3A genomic library from this strain was constructed and screened for repetitive DNA elements. An insert of 438 bp (Rep1) from one library clone hybridized to multiple chromosomal DNA fragments resolved electrophoretically after digestion with BamHI, HindIII, and MfeI. A single oligonucleotide primer, designated iRepl, was designed to generate multiple PCR amplicons of various electrophoretic mobilities in a PCR typing method. The method distinguished strains belonging to the eight pathogenic and three saprophytic species of the genus Leptospira. Clinical isolates obtained during urban epidemics between 1996 and 1998 in Salvador, Brazil, were analyzed by this PCR method. Although the iRep1 primer was unable to discriminate strains among L. interrogans serovar copenhageni isolates, it was able to differentiate strains belonging to different species and serogroups of Leptospira identified in Salvador. This PCR-based method may provide a faster and less expensive alternative to serologic tests used in reference laboratories.
dc.formatapplication/pdf
dc.languageeng
dc.publisherAmerican Society for Microbiology
dc.rightsopen access
dc.subjectLeptospira interrogans/classificação
dc.subjectLeptospira interrogans/genética
dc.subjectLeptospira/classificação
dc.subjectRepetições de Trinucleotídeos/genética
dc.subjectDoença de Weil/microbiologia
dc.subjectAnimais
dc.subjectSequência de Bases
dc.subjectDNA Bacteriano/análise
dc.subjectDNA Bacteriano/genética
dc.subjectDosagem de Genes
dc.subjectBiblioteca Genômica
dc.subjectHumanos
dc.subjectLeptospira/genética
dc.subjectLeptospira interrogans/isolamento & purificação
dc.subjectDados de Sequência Molecular
dc.subjectReação em Cadeia da Polimerase/métodos
dc.subjectAnálise de Sequência de DNA
dc.subjectSorotipagem
dc.subjectDoença de Weil/epidemiologia
dc.titleIdentification of new repetitive element in Leptospira interrogans serovar copenhageni and its application to PCR-based differentiation of Leptospira serogroups.
dc.typeArticle


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