Chitinolytic Activity in Viable Spores of Encephalitozoon Species

dc.creatorSchottelius, J.
dc.creatorHünger, F.
dc.creatorSchüler, T. H.
dc.creatorCosta, S. C. Gonçalves da
dc.date2020-10-20T18:19:57Z
dc.date2020-10-20T18:19:57Z
dc.date2000
dc.date.accessioned2023-09-26T20:31:38Z
dc.date.available2023-09-26T20:31:38Z
dc.identifierSCHOTTELIUS, J. et al. Chitinolytic Activity in Viable Spores of Encephalitozoon Species. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 95, n. 5, p. 701-705, Sept./Oct. 2000.
dc.identifier0074-0276
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/44091
dc.identifier10.1590/S0074-02762000000500015
dc.identifier1678-8060
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8858845
dc.descriptionBy employing 4-methylumbelliferyl-beta-D-NN’,N”-triacetylchitotriose substrate in a semi quantitative assay, chitinolytic activity in viable spores of Encephalitozoon cuniculi and E. intestinalis was detected and dependence on reaction time, spore concentration, concentration of substrate and temperature were demonstrated. It was possible to block the chitinolytic activity by chitin hydrolysate. By incubation at 80°C for 10 min or at 55°C for 20 min the spores were loosing the chitinolytic activity. Incubation of the spores in trypsin reduced the chitinolytic activity. Cellulase activity could not be detected.
dc.formatapplication/pdf
dc.languageeng
dc.publisherFundação Oswaldo Cruz. Instituto Oswaldo Cruz.
dc.rightsopen access
dc.subjectMicrospora
dc.subjectInibição
dc.subjectCellulase
dc.subjectAtividade de Chitinase
dc.subjectMicrospora
dc.subjectCellulase
dc.subjectChitinase activity
dc.subjectInhibition
dc.titleChitinolytic Activity in Viable Spores of Encephalitozoon Species
dc.typeArticle


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