Characterization of subpopulations (clones and subclones) of the 21 SF strain of Trypanosoma cruzi after long lasting maintenance in the laboratory.

dc.creatorCampos, Rozalia Mendes Figueira
dc.creatorAndrade, Sonia Gumes
dc.date2012-10-16T21:19:24Z
dc.date2012-10-16T21:19:24Z
dc.date1996
dc.date.accessioned2023-09-26T20:31:21Z
dc.date.available2023-09-26T20:31:21Z
dc.identifierCAMPOS, R.M.; ANDRADE, S.G. Characterization of subpopulations (clones and subclones) of the 21 SF strain of Trypanosoma cruzi after long lasting maintenance in the laboratory. Memórias do Instituto Oswaldo Cruz, v. 91, n. 6, p. 795-800, nov.-dec. 1996.
dc.identifier0074-0276
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/5676
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8858741
dc.descriptionSeveral studies have shown a clonal structure of Trypanosoma cruzi and its possible correlation with the behavioral heterogeneity of the parasite strains. In the present study, the 21 SF strain, that have been maintained in laboratory by successive passages in mice, for more than 15 years, showing a stability of biological and isoenzymic characteristics has been cloned, with the objective of establishing the characters of its clones and subclones. With the technique of isolation of a single parasite from the blood of infected mice, 5 clones and 14 subclones have been obtained. After four passages into mice, inoculum of 10(5) was obtained for each clone and subclone and inoculated into mice weighing 10 to 12 g. These were used for the study of the biological behavior of the clones: evolution of parasitemia, morphology of blood forms and host mortality. For isoenzymic characterization, the clones and subclones were analyzed for ALAT, ASAT, GPI and PGM enzymes. Results have shown that the 5 clones and the 14 subclones disclosed a biological behavior similar to the parental strain, with minor variability of the parasitemic profiles and also the same isoenzymic patterns. These results confirm the stability of the 21 SF strain and indicate a clonal homogeneity of its populations. This is compatible with the hypothesis that the T. cruzi strains represent an equilibrium of either homogenous or heterogeneous populations.
dc.formatapplication/pdf
dc.languageeng
dc.rightsopen access
dc.subjectTrypanosoma cruzi
dc.subjectStrain
dc.subjectClones
dc.subjectBiological characterization
dc.subjectZymodeme
dc.subjectTrypanosoma cruzi/crescimento & desenvolvimento
dc.subjectAnimais
dc.subjectClonagem de Organismos
dc.subjectGenética Populacional
dc.subjectCamundongos
dc.subjectParasitemia
dc.subjectFatores de Tempo
dc.subjectTrypanosoma cruzi/enzimologia
dc.subjectTrypanosoma cruzi/genética
dc.titleCharacterization of subpopulations (clones and subclones) of the 21 SF strain of Trypanosoma cruzi after long lasting maintenance in the laboratory.
dc.typeArticle


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