Trypanosoma cruzi: requirements for induction and maintenance of protective immunity conferred by immunization

dc.creatorPaiva, Claudia Neto
dc.creatorPyrrho, Alexandre dos Santos
dc.creatorRibeiro, Liane de Jesus
dc.creatorGonçalves, Renata
dc.creatorCosta, Deise de Andrade
dc.creatorAraujo-Jorge, Tania C.
dc.creatorSoares, Milena Botelho Pereira
dc.creatorGattass, Cerli Rocha
dc.date2015-04-07T18:05:06Z
dc.date2015-04-07T18:05:06Z
dc.date2002
dc.date.accessioned2023-09-26T20:31:14Z
dc.date.available2023-09-26T20:31:14Z
dc.identifierPAIVA, Claudia Neto et al. Trypanosoma cruzi: requirements for induction and maintenance of protective immunity conferred by immunization. Experimental Parasitology, v. 102, n. 2, p. 89-98, 2002.
dc.identifier0014-4894
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/9912
dc.identifier10.1016/S0014-4894(03)00025-0
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8858699
dc.descriptionImmunization with CL-14-trypomastigotes generates efficient humoral and cellular responses against infective challenge. Herein, we investigated the relevance of these mechanisms in vivo. Immunization with live CL-14-trypomastigotes protected only part of b2m = mice but efficiently protected perforin-knockout mice. Fixed CL-14-trypomastigotes could successfully immunize BALB/c, though live trypomastigotes lowered the requirements for doses and time intervals. Post-immune depletion of CD4 or CD8 subsets did not affect protection conferred by immunization, but switched the production of anti-Trypanosoma cruzi antibodies to IgG2a. Sublethal irradiation partially broke the resistance of immune mice, leading to development of late parasitemia. Passive serum transfer from immune mice conferred protection to na€ıve mice. Our results indicate that presentation of cytosolic antigens by MHC class I molecules is involved in the generation of immunity and suggest that the humoral response contributes to a great extent to keep CL-14-immunized mice protected against infective challenge.
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsopen access
dc.subjectDoença de Chagas/prevenção & controle
dc.subjectTrypanosoma cruzi/imunologia
dc.subjectImunização
dc.subjectAnimais
dc.subjectAnticorpos Antiprotozoários/sangue
dc.subjectTransplante de Medula Óssea/imunologia
dc.subjectLinfócitos T CD4-Positivos/imunologia
dc.subjectDoença de Chagas/imunologia
dc.subjectFeminino
dc.subjectImunidade Celular
dc.subjectImunização Passiva
dc.subjectTecido Linfoide/imunologia
dc.subjectMasculino
dc.subjectGlicoproteínas de Membrana/genética
dc.subjectCamundongos
dc.subjectCamundongos Endogâmicos BALB C
dc.subjectCamundongos Knockout
dc.subjectParasitemia/imunologia
dc.titleTrypanosoma cruzi: requirements for induction and maintenance of protective immunity conferred by immunization
dc.typeArticle


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