| dc.creator | De Simone, S. G. | |
| dc.creator | Netto, C. C. | |
| dc.creator | Silva, F. P. | |
| dc.date | 2020-01-16T12:29:06Z | |
| dc.date | 2020-01-16T12:29:06Z | |
| dc.date | 2006 | |
| dc.date.accessioned | 2023-09-26T20:31:13Z | |
| dc.date.available | 2023-09-26T20:31:13Z | |
| dc.identifier | DE SIMONE, S. G. de; NETTO, C. C.; SILVA JR., F. P. Simple affinity chromatographic procedure to purify -galactoside binding lectins. Journal of Chromotherapy B, v. 838, p. 135-138, 2006. | |
| dc.identifier | 1570-0232 | |
| dc.identifier | https://www.arca.fiocruz.br/handle/icict/39250 | |
| dc.identifier | 10.1016/j.jchromb.2006.02.036 | |
| dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/8858697 | |
| dc.description | Affinity chromatography based on the commercial resin Sepharose CL-6B was used to isolate new C1-beta-type lectins from crude preparations of snake venoms (Bothrops jararaca, Bothrops jararacussu, Bothrops newiedi, Bothrops moojeni, Lachesis muta rhombeata). Most of the C-type lectins could be eluted with almost 100% recovery using the competitor isopropyl-beta-D-thiogalactoside (IPTG) or through Ca2+ sequestration with EDTA. The lectin yield varied considerably among the different snake species, but B. newiedi venom was a particularly rich source of lectin, retaining 2.7 mg of lectin by milliliter of resin in saturating conditions. C1-alpha-lectins from Crotalus durisus terrificus venom, from the jack fruit (jacalin) and from bread fruit seeds extract (frutalin) had no affinity, either with or without Ca2+ added, for Sepharose CL-6B, showing that the resin is specific for C1-beta-type lectins. Sepharose CL-6B used as galactose-affinity chromatography provides a simple and fast method for isolating C-type beta-galactoside binding lectins from crude sample preparations. | |
| dc.description | 2022-01-01 | |
| dc.format | application/pdf | |
| dc.language | eng | |
| dc.publisher | Elsevier | |
| dc.rights | restricted access | |
| dc.subject | Cromatografia de afinidade | |
| dc.subject | Lectinas | |
| dc.subject | Lectinas de ligação ao galactósido do tipo C | |
| dc.subject | C-type -galactoside binding lectins | |
| dc.subject | Affinity chromatography | |
| dc.subject | Lectins | |
| dc.title | Simple affinity chromatographic procedure to purify beta-galactoside binding lectins | |
| dc.type | Article | |
