| dc.creator | Diniz-Mendes, L. | |
| dc.creator | Paula, V. S. de | |
| dc.creator | Luz, Sérgio Luiz Bessa | |
| dc.creator | Niel, C. | |
| dc.date | 2011-04-21T14:35:23Z | |
| dc.date | 2011-04-21T14:35:23Z | |
| dc.date | 2008 | |
| dc.date.accessioned | 2023-09-26T20:31:00Z | |
| dc.date.available | 2023-09-26T20:31:00Z | |
| dc.identifier | DINIZ-MENDES, L. et al. High prevalence of human Torque teno virus in streams crossing the city of Manaus, Brazilian Amazon. Journal of Applied Microbiology, v. 105, n. 1, p. 51-58, 2008. | |
| dc.identifier | 1364-5072 | |
| dc.identifier | https://www.arca.fiocruz.br/handle/icict/1927 | |
| dc.identifier | 10.1111/j.1365-2672.2007.03720.x | |
| dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/8858615 | |
| dc.description | Aims: Torque teno virus (TTV) is a human DNA virus chronically infecting
most healthy individuals worldwide and can be transmitted by faecal–oral
route. The occurrence of TTV was evaluated in the streams crossing the city of
Manaus (Brazilian Amazon) over a 1-year period, four times a year.
Methods and Results: Fifty-two water samples were collected from 13 different
locations. Viruses were concentrated from two litres of water by adsorption to
negative membrane filters followed by ultrafiltration. TTV DNA was detected
by PCR assays designed to detect all five TTV genomic groups. By conventional
PCR, 19 ⁄ 52 (37%) samples were positive. By real-time PCR, TTV DNA could
be detected in 48 ⁄ 52 (92%) samples. Viral loads ranged from 1300 to 746 000
genome equivalent per 100 ml of river water. Eleven distinct nucleotide
sequences were obtained.
Conclusions: Our results show the wide distribution and diversity of TTV
among Manaus urban micro basins.
Significance and Impact of the Study: The data presented here may contribute
to substantiate TTV as a sensitive indicator of human contamination. | |
| dc.format | application/pdf | |
| dc.language | eng | |
| dc.rights | restricted access | |
| dc.subject | Anellovirus | |
| dc.subject | nucleotide sequencing | |
| dc.subject | real-time PCR | |
| dc.subject | river water | |
| dc.subject | Torque teno virus | |
| dc.subject | viral indicator | |
| dc.title | High prevalence of human Torque teno virus in streams crossing the city of Manaus, Brazilian Amazon | |
| dc.type | Article | |
