Detection of mixed populations of wild-type and YMDD hepatitis B variants by pyrosequencing in acutely and chronically infected patients

dc.creatorMello, Francisco C. A.
dc.creatorLago, Bárbara V.
dc.creatorLewis-Ximenez, Lia L.
dc.creatorFernandes, Carlos A.
dc.creatorGomes, Selma A.
dc.date2017-01-17T16:03:07Z
dc.date2017-01-17T16:03:07Z
dc.date2012
dc.date.accessioned2023-09-26T20:30:52Z
dc.date.available2023-09-26T20:30:52Z
dc.identifierMELLO, Francisco C. A. et al. Detection of mixed populations of wild-type and YMDD hepatitis B variants by pyrosequencing in acutely and chronically infected patients. BMC Microbiology, v.12, n.96, 9p, 2012.
dc.identifier1471-2180
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/16871
dc.identifier10.1186/1471-2180-12-96
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8858567
dc.descriptionLamivudine (LAM) is associated with the highest known rate of resistance mutations among nucleotide analogs used to treat chronic hepatitis B virus (HBV) infection. Despite this, LAM continues in widespread use, especially in combination therapies. The primary LAM resistance mutation (rtM204V/I) occurs in the YMDD motif of HBV polymerase. The aim of this study was to characterize Brazilian HBV isolates from acute and chronic cases by direct sequencing, and to identify HBV quasispecies in the YMDD motif using a pyrosequencing method capable of detecting single-nucleotide polymorphisms. HBV DNA from serum samples of 20 individuals with acute HBV infection and 44 with chronic infection undergoing antiviral therapies containing LAM were analyzed by direct sequencing and pyrosequencing methods.
dc.formatapplication/pdf
dc.languageeng
dc.publisherBioMed Central
dc.rightsopen access
dc.subjectLamivudina
dc.subjectTratamento
dc.subjectInfecção crônica pelo vírus da hepatite B (HBV)
dc.subjectTerapias antivirais
dc.subjecthepatitis B
dc.subjecttreatment
dc.subjectchronic hepatitis B virus (HBV) infection
dc.subjectYMDD variants
dc.subjectantiviral therapies
dc.subjectLamiduvine
dc.titleDetection of mixed populations of wild-type and YMDD hepatitis B variants by pyrosequencing in acutely and chronically infected patients
dc.typeArticle


Este ítem pertenece a la siguiente institución