Co-localization of mutant p53 and amyloid-like protein aggregates in breast tumors

dc.creatorLevy, Claudia B.
dc.creatorStumbo, Ana C.
dc.creatorBom, Ana P.D. Ano
dc.creatorPortari, Elisabeth A.
dc.creatorCarneiro, Yraima
dc.creatorSilva, Jerson L.
dc.creatorMoura-Gallo, Claudia V. De
dc.date2015-01-08T12:49:57Z
dc.date2015-01-08T12:49:57Z
dc.date2011
dc.date.accessioned2023-09-26T20:30:41Z
dc.date.available2023-09-26T20:30:41Z
dc.identifierLEVY, Claudia B. et al. Co-localization of mutant p53 and amyloid-like protein aggregates in breast tumors. The International Journal of Biochemistry & Cell Biology., v. 43, p. 60-64, 2011
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/9347
dc.identifier10.1016/j.biocel.2010.10.017
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8858498
dc.descriptionP53 isoneof themost important tumor suppressorproteins inhumancancers.Mutations in theTP53gene are common features of malignant tumors and normally correlate to a more aggressive disease. In breast cancer, these gene alterations are present in approximately 20% of cases and are characteristically of missense type. In the present work we describeTP53mutations in breast cancer biopsies and investigate whether wild and mutant p53 participate in protein aggregates formation in these breast cancer cases. We analyzed 88 biopsies from patients residing in the metropolitan area of Rio de Janeiro, andperformed TP53mutation screening using direct sequencing of exons 5–10. Seventeen mutations were detected, 12 of them were of missense type, 2 nonsenses, 2 deletions and 1 insertion. The presence ofTP53mutation was highly statistically associated to tumor aggressiveness of IDC cases, indicatedhere by ElstonGrade III (p<0.0001). Paraffin embedded breast cancer tissues were analyzed for the presence of p53 aggregates through immunofluorescenceco-localizationassay, usinganti-aggregateprimaryantibodyA11, andanti-p53. Our results show that mutant p53 co-localizes with amyloid-like protein aggregates, depending on mutation type, suggesting that mutant p53 may form aggregates in breast cancer cells,in vivo.
dc.formatapplication/pdf
dc.languageeng
dc.rightsrestricted access
dc.subjectp53
dc.subjectBreast Cancer
dc.subjectMutation
dc.subjectProtein Aggregate
dc.subjectAntibody A11
dc.subjectGenes p53
dc.subjectNeoplasias da Mama
dc.subjectMutação
dc.subjectAnexinas
dc.titleCo-localization of mutant p53 and amyloid-like protein aggregates in breast tumors
dc.typeArticle


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