Deficiency of glycerol-3-phosphate acyltransferase 1 decreases triacylglycerol storage and induces fatty acid oxidation in insect fat body

dc.creatorBezerra, Michele Alves
dc.creatorRamos, Isabela B.
dc.creatorPaula, Iron F. de
dc.creatorMonteiro, Clarissa M. Maya
dc.creatorKlett, Eric L.
dc.creatorColeman, Rosalind A.
dc.creatorGondim, Katia C.
dc.date2017-07-06T11:56:02Z
dc.date2017-07-06T11:56:02Z
dc.date2017
dc.date.accessioned2023-09-26T20:30:11Z
dc.date.available2023-09-26T20:30:11Z
dc.identifierBEZERRA, Michele Alves; et al. Deficiency of glycerol-3-phosphate acyltransferase 1 decreases triacylglycerol storage and induces fatty acid oxidation in insect fat body. Biochimica et Piophysica Acta, v.1862, p.324-336, 2017.
dc.identifier1388-1981
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/19735
dc.identifier10.1016/j.bbalip.2016.12.004
dc.identifier0006-3002
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8858315
dc.descriptionGlycerol-3-phosphate acyltransferases (GPAT) catalyze the initial and rate-limiting step for the de novo synthesis of triacylglycerol (TAG). Four mammalian GPAT isoforms have been identified: the mitochondria-associated GPAT1 and 2, and the endoplasmic reticulum (ER)-associated GPAT3 and 4. In the insect Rhodnius prolixus, a vector of Chagas' disease, we previously predicted a mitochondrial-like isoform (RhoprGPAT1) from genomic data. In the current study, we clone the RhoprGPAT1 coding sequence and identify an ER-associated GPAT (RhoprGPAT4) as the second isoform in the insect. RhoprGPAT1 contributes 15% of the total GPAT activity in anterior midgut, 50% in posterior midgut and fat body, and 70% in the ovary. The RhoprGpat1 gene is the predominant transcript in the midgut and fat body. To evaluate the physiological relevance of RhoprGPAT1, we generate RhoprGPAT1-deficient insects. The knockdown of RhoprGpat1 results in 50% and 65% decrease in TAG content in the posterior midgut and fat body, respectively. RhoprGpat1-deficient insects also exhibits impaired lipid droplet expansion and a 2-fold increase in fatty acid β-oxidation rates in the fat body. We propose that the RhoprGPAT1 mitochondrial-like isoform is required to channel fatty acyl chains towards TAG synthesis and away from β-oxidation. Such a process is crucial for the insect lipid homeostasis.
dc.description2030-01-01
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsrestricted access
dc.subjectLipídios
dc.subjectBeta-oxidação
dc.subjectMetabolismo dos ácidos gordurosos
dc.subjectSíntese de triacilglicerol
dc.subjectOxidação de ácido gordo
dc.subjectFisiologia dos Insetos
dc.subjectBeta-oxidation
dc.subjectFatty acid metabolism
dc.subjectLipids
dc.subjectTriacylglycerol synthesis
dc.subjectFatty acid oxidation
dc.subjectInsect physiology
dc.titleDeficiency of glycerol-3-phosphate acyltransferase 1 decreases triacylglycerol storage and induces fatty acid oxidation in insect fat body
dc.typeArticle


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