Leishmania infantum virulence factor A2 protein: linear B-cell epitope mapping and identification of three main linear B-cell epitopes in vaccinated and naturally infected dogs

dc.creatorCampos, Monique Paiva
dc.creatorFigueiredo, Fabiano Borges
dc.creatorMorgado, Fernanda Nazaré
dc.creatorRenzetti, Alinne Rangel dos Santos
dc.creatorSouza, Sara Maria Marques de
dc.creatorPereira, Sandro Antônio
dc.creatorRodrigues-da-Silva, Rodrigo Nunes
dc.creatorLima-Junior, Josué da Costa
dc.creatorLuca, Paula Mello de
dc.date2019-02-11T11:01:35Z
dc.date2019-02-11T11:01:35Z
dc.date2018
dc.date.accessioned2023-09-26T20:30:05Z
dc.date.available2023-09-26T20:30:05Z
dc.identifierCAMPOS, Monique Paiva et al. Leishmania infantum virulence factor A2 protein: linear B-cell epitope mapping and identification of three main linear B-cell epitopes in vaccinated and naturally infected dogs. Frontiers in Immunology, v. 9, p. 1-11, July 2018.
dc.identifier1664-3224
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/31525
dc.identifier10.3389/fimmu.2018.01690
dc.identifier1664-3224
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8858279
dc.descriptionInclui errata.
dc.descriptionIn Brazil, canine visceral leishmaniasis (CVL) is caused by Leishmania infantum, presenting a broad spectrum of clinical manifestations. Dogs are the main parasite reservoir in urban areas and canine cases precede human infection. Currently, A2 protein-based Leish-Tec® vaccine is the only vaccine commercially available against CVL in Brazil. Considering that the main screening and confirmatory tests of canine infection are serological, it is possible that the antibody response elicited after vaccination interfere with diagnosis, leading to the inability to distinguish between vaccinated and infected animals. In order to identify the specific B-cell response induced after vaccination, A2 protein sequence was screened for main linear B-cell epitopes using in silico prediction (Bepipred) and immunological confirmation by enzyme-linked immunosorbent assay (ELISA). Three amino acid sequences were described as potential B-cell epitopes (PP16-SAEPHKAAVDV, SV11-PQSVGGPLSVGPQSVGP, and VQ34-VGPLSVGPQSVGPLSVGPLSVGPQAVGPLSVGPQ). Specific IgG ELISAs were performed in sera of 12 immunized dogs living in non-endemic areas, followed for up to 1 year after immunization. The results were compared with those obtained in a group of 10 symptomatic and 10 asymptomatic CVL dogs. All predicted epitopes were confirmed as linear B-cell epitopes broadly recognized by sera from studied dogs. Total IgG ELISAs demonstrated distinct patterns of response between peptides in the immunized and CVL groups. VQ34 peptide was recognized by the majority of sera from vaccinated and symptomatic dogs, and increases after vaccination. PP16 induced low levels of specific IgG that increased 1 year after immunization. Interestingly, a low frequency of reactivity was found against SV11 in naturally infected dogs (symptomatic and asymptomatic), while 83.3% of vaccinated dogs presented positive responses 1 year after immunization. The two animals in the vaccinated group that did not respond to SV11 1 year after immunization presented positive serology both 30 days and 6 months after immunization. In summary, we identified three main linear B-cell epitopes in A2-based vaccine. Moreover, the humoral response against SV11 presented marked differences between infected and Leish-Tec® vaccinated dogs and should be further investigated, in large trials, to confirm its potential as a serological marker able to distinguish between infected and vaccinated dogs.
dc.formatapplication/pdf
dc.formatapplication/pdf
dc.languageeng
dc.publisherFrontiers Media
dc.rightsopen access
dc.subjectCanine visceral leishmaniasis
dc.subjectVaccines
dc.subjectA2 protein
dc.subjectSerology test
dc.subjectEpitope mapping
dc.subjectEpitope prediction
dc.titleLeishmania infantum virulence factor A2 protein: linear B-cell epitope mapping and identification of three main linear B-cell epitopes in vaccinated and naturally infected dogs
dc.typeArticle


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