Evaluation of murine monoclonal antibodies targeting different epitopes of the hepatitis B virus surface antigen by using immunological as well as molecular biology and biochemical approaches

dc.creatorVianna, Carlos Otávio Alves
dc.creatorMouta Junior, Sergio da Silva e
dc.creatorMartins, Maria da Glória Teixeira
dc.creatorBatoreu, Nadia Maria
dc.creatorQueiroz, João Luiz
dc.creatorGomes, Selma Andrade
dc.creatorGóes, Ana Carolina Magalhães de Andrade
dc.creatorArmoa, Geraldo Rodrigues Garcia
dc.creatorMarques, Carlos Humberto
dc.creatorMoraes, Márcia Terezinha Baroni de
dc.date2020-02-06T11:38:04Z
dc.date2020-02-06T11:38:04Z
dc.date2006
dc.date.accessioned2023-09-26T20:29:56Z
dc.date.available2023-09-26T20:29:56Z
dc.identifierVIANNA, Carlos Otávio Alves et al. Evaluation of murine monoclonal antibodies targeting different epitopes of the hepatitis B virus surface antigen by using immunological as well as molecular biology and biochemical approaches. Journal of Immunological Methods, v. 313, p. 38-47, 2006.
dc.identifier0022-1759
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/39759
dc.identifier10.1016/j.jim.2006.03.008
dc.identifier1872-7905
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8858226
dc.descriptionThe hepatitis B virus surface protein (HBsAg) displays the major B cells antigenic determinants that can induce protective immunity and prevent the hepatitis B virus (HBV) infection, a major health problem. A panel of murine monoclonal antibodies against the HBsAg (MAb anti-HBs), raised after mice immunization with a pool of plasma of hepatitis chronic carriers, has been established. Mainly using simple immunological tools such as enzyme-linked immunosorbent assay (ELISA) and Western blot analysis, we could trace the location of the epitopes on the HBsAg determinants. We also report the use of two specific methodology approaches based on molecular biology and biochemical techniques such as, respectively, cloning and expression of preS1 major neutralizing epitope of the HBsAg in Escherichia coli and ELISA accomplished to chemical reduction with dithiothreitol (DTT), which were able to complete the MAb anti-HBs characterization. Our results showed that the majority of the MAbs anti-HBs were directed to the HBV common determinant a. One MAb recognizes a discontinuous epitope present in all forms of the HBsAg when evaluated by Western blot.
dc.description2025-01-01
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsrestricted access
dc.subjectAnticorpo monoclonal
dc.subjectAntígeno de superfície da hepatite B
dc.subjectMonoclonal antibody
dc.subjectHBsAg
dc.subjectWestern blot
dc.subjectELISA
dc.subjecta determinant
dc.titleEvaluation of murine monoclonal antibodies targeting different epitopes of the hepatitis B virus surface antigen by using immunological as well as molecular biology and biochemical approaches
dc.typeArticle


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