Evaluation of na Enzyme Linked Immunosorbent Assay (ELISA) using purified, deglycosylated histoplamin for different clinical manifestations of histoplamosis

dc.creatorGuimarães, Allan Jefferson
dc.creatorPizzini, Claudia Vera
dc.creatorAlmeida, Marcos de Abreu
dc.creatorPeralta, José Mauro
dc.creatorNosanchuk, Joshua Daniel
dc.creatorZancope-Oliveira, Rosely Maria
dc.date2019-12-10T13:52:40Z
dc.date2019-12-10T13:52:40Z
dc.date2010
dc.date.accessioned2023-09-26T20:29:54Z
dc.date.available2023-09-26T20:29:54Z
dc.identifierGUIMARÃES, Allan Jefferson et al. Evaluation of an enzyme-linked immunosorbent assay using purified, deglycosylated histoplasmin for different clinical manifestations of histoplasmosis. Microbiology Research, v. 1, n. 1, p. 1-21, 2010.
dc.identifier2036-7481
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/37747
dc.identifier10.4081/mr.2010.e2
dc.identifier2036-7473
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8858213
dc.descriptionDiagnosis of invasive fungal diseases remains problematic, especially in undeveloped countries. We have developed an enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies to Histoplasma capsulatum using metaperiodate treated purified histoplasmin (ptHMIN). Our ELISA was validated comparing sera from patients with histoplasmosis, related mycoses, and healthy individuals. The overall test specificity was 96%, with sensitivities of 100% (8/8) in acute disease, 90% (9/10) in chronic disease, 89% (8/9) in disseminated infection in individuals without HIV infection, 86% (12/14) in disseminated disease in the setting of HIV infection and 100% (3/3) in mediastinal histoplasmosis. These parameters are superior to the use of untreated histoplasmin in diagnostic ELISAs. The high specificities, sensitivities, and simplicity of our ELISA support further development of a deglycosylated HMIN ELISA for clinical use and for monitoring the humoral immune response during therapy in patients with chronic and disseminated histoplasmosis.
dc.formatapplication/pdf
dc.languageeng
dc.publisherPAGEpress
dc.rightsopen access
dc.subjectELISA
dc.subjectAntibody detection
dc.subjectHistoplasmosis
dc.subjectSerological diagnosis
dc.titleEvaluation of na Enzyme Linked Immunosorbent Assay (ELISA) using purified, deglycosylated histoplamin for different clinical manifestations of histoplamosis
dc.typeArticle


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