A preliminary molecular epidemiologic study using analysis of variable number of tandem repeats of Acinetobacter baumannii OXA-23 producing strains isolated from hospitals in Rio de Janeiro State, Brazil

Estudo epidemiológico molecular preliminar usando a análise de número variável de repetições em tandem (VNTR) de cepas de Acinetobacter baumannii produtoras de OXA-23 isoladas em hospitais no Rio de Janeiro, Brasil;
Estudio epidemiológico molecular preliminar usando el análisis de número variable en tándem de repeticiones (VNTR) de cepas de Acinetobacter baumannii productoras de OXA-23 aisladas en hospitales de Rio de Janeiro, Brasil

dc.creatorCarramachi, Isabel Nogueira
dc.creatorCarvalho, Karyne Rangel
dc.creatorCruz, Jéssica Gonçalves Vieira da
dc.creatorZahner, Viviane
dc.date2015-06-10T13:55:19Z
dc.date2015-06-10T13:55:19Z
dc.date2014
dc.date.accessioned2023-09-26T20:29:28Z
dc.date.available2023-09-26T20:29:28Z
dc.identifierCARRAMACHI, Isabel Nogueira et al. A preliminary molecular epidemiologic study using analysis of variable number of tandem repeats of Acinetobacter baumannii OXA-23 producing strains isolated from hospitals in Rio de Janeiro State, Brazil. Rev Pan-Amaz Saude, v.5, n.2, Ananindeua jun. 2014
dc.identifier2176-6223
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/10762
dc.identifier10.5123/S2176-62232014000200008
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8858068
dc.descriptionThe Gram-negative multidrug-resistant (MDR) bacterium Acinetobacter baumannii constitutes a serious cause of nosocomial infections in Brazilian hospitals. A panel of 36 strains, belonging to five pre-determined pulsed-field gel electrophoresis (PFGE) genotypes from four different hospitals in the City of Rio de Janeiro, Rio de Janeiro State, Brazil, was submitted to simple agarose gel electrophoresis to determine the variation of nine variable number tandem repeats (VNTR) band profiles. Based on published data VNTR were classified in two categories, long (L) and short (S) repeats with different evolutionary implications. The results demonstrated the superior discrimination of VNTR over PFGE. The VNTR Abaum_3002 marker was the least discriminatory with only one allele, while VNTR10 and Abaum_2240 each presented four alleles. The use of a combination of the nine VNTR resulted in a refined genotyping tool that provides valuable epidemiological information.
dc.formatapplication/pdf
dc.languageeng
dc.publisherInstituto Evandro Chagas/SVS/MS
dc.rightsopen access
dc.subjectAcinetobacter baumannii
dc.subjectVNTR
dc.subjectPublic Health
dc.subjectEpidemiology
dc.subjectSaúde Pública
dc.subjectEpidemiologia
dc.subjectAcinetobacter baumannii
dc.subjectRepetições Minissatélites
dc.titleA preliminary molecular epidemiologic study using analysis of variable number of tandem repeats of Acinetobacter baumannii OXA-23 producing strains isolated from hospitals in Rio de Janeiro State, Brazil
dc.titleEstudo epidemiológico molecular preliminar usando a análise de número variável de repetições em tandem (VNTR) de cepas de Acinetobacter baumannii produtoras de OXA-23 isoladas em hospitais no Rio de Janeiro, Brasil
dc.titleEstudio epidemiológico molecular preliminar usando el análisis de número variable en tándem de repeticiones (VNTR) de cepas de Acinetobacter baumannii productoras de OXA-23 aisladas en hospitales de Rio de Janeiro, Brasil
dc.typeArticle


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