Performance Assessment of Four Chimeric Trypanosoma cruzi Antigens Based on Antigen-Antibody Detection for Diagnosis of Chronic Chagas Disease

dc.creatorSantos, Fred Luciano Neves
dc.creatorCeledon, Paola Alejandra Fiorani
dc.creatorZanchin, Nilson Ivo Tonin
dc.creatorBrasil, Tatiana de Arruda Campos
dc.creatorFoti, Leonardo
dc.creatorSouza, Wayner Vieira de
dc.creatorSilva, Edmilson Domingos
dc.creatorGomes, Yara de Miranda
dc.creatorKrieger, Marco Aurélio
dc.date2017-06-05T18:44:44Z
dc.date2017-06-05T18:44:44Z
dc.date2016
dc.date.accessioned2023-09-26T20:29:13Z
dc.date.available2023-09-26T20:29:13Z
dc.identifierSANTOS, F. L. N. et al. Performance Assessment of Four Chimeric Trypanosoma cruzi Antigens Based on Antigen-Antibody Detection for Diagnosis of Chronic Chagas Disease. PLOS ONE, v. 11, n. 8, p. e0161100, 12 ago. 2016.
dc.identifier1932-6203
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/19302
dc.identifier10.1371/journal.pone.0161100
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8857971
dc.descriptionThe performance of serologic tests in chronic Chagas disease diagnosis largely depends on the type and quality of the antigen preparations that are used for detection of anti-Trypanosoma cruzi antibodies. Whole-cell T. cruzi extracts or recombinant proteins have shown variation in the performance and cross-reactivity. Synthetic chimeric proteins comprising fragments of repetitive amino acids of several different proteins have been shown to improve assay performances to detect Chagasic infections. Here, we describe the production of four chimeric T. cruzi proteins and the assessment of their performance for diagnostic purposes. Circular Dichroism spectra indicated the absence of well-defined secondary structures, while polydispersity evaluated by Dynamic Light Scattering revealed only minor aggregates in 50 mM carbonate-bicarbonate (pH 9.6), demonstrating that it is an appropriate buffering system for sensitizing microplates. Serum samples from T. cruzi-infected and non-infected individuals were used to assess the performance of these antigens for detecting antibodies against T. cruzi, using both enzyme-linked immunosorbent assay and a liquid bead array platform. Performance parameters (AUC, sensitivity, specificity, accuracy and J index) showed high diagnostic accuracy for all chimeric proteins for detection of specific anti-T. cruzi antibodies and differentiated seropositive individuals from those who were seronegative. Our data suggest that these four chimeric proteins are eligible for phase II studies.
dc.formatapplication/pdf
dc.languageeng
dc.rightsopen access
dc.subjectTrypanosoma cruzi
dc.subjectDoença de Chagas
dc.subjectTestes Sorológicos
dc.subjectDiagnóstico
dc.subjectAnticorpos, protozoários / imunologia
dc.subjectAntígenos , protozoários / imunologia
dc.subjectDoença de Chagas / diagnóstico
dc.subjectEnsaio de Imunoadsorção Enzimática / métodos
dc.subjectTrypanosoma cruzi / imunologia
dc.titlePerformance Assessment of Four Chimeric Trypanosoma cruzi Antigens Based on Antigen-Antibody Detection for Diagnosis of Chronic Chagas Disease
dc.typeArticle


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