Triton X-100 solubilized bone matrix-induced alkaline phosphatase

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorPizauro, João M.
dc.creatorCurti, Carlos
dc.creatorCiancaglini, Pietro
dc.creatorLeone, Francisco A.
dc.date2014-05-27T03:23:31Z
dc.date2016-10-25T18:12:11Z
dc.date2014-05-27T03:23:31Z
dc.date2016-10-25T18:12:11Z
dc.date1987-12-01
dc.date.accessioned2017-04-06T00:41:09Z
dc.date.available2017-04-06T00:41:09Z
dc.identifierComparative Biochemistry and Physiology -- Part B: Biochemistry and, v. 87, n. 4, p. 921-926, 1987.
dc.identifier0305-0491
dc.identifierhttp://hdl.handle.net/11449/63818
dc.identifierhttp://acervodigital.unesp.br/handle/11449/63818
dc.identifier10.1016/0305-0491(87)90413-5
dc.identifier2-s2.0-0023073346
dc.identifierhttp://dx.doi.org/10.1016/0305-0491(87)90413-5
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/885783
dc.description1. 1. Solubilized and membrane-bound alkaline phosphatase showed Michaelis-Menten behavior in a wide range of different substrate concentrations. 2. 2. Membrane-bound alkaline phosphatase has a molecular weight of 130,000 and its minimum active configuration comprises two identical subunits of about 65,000. 3. 3. The two forms of the enzyme behave similarly with respect to NaCl, urea and guanidine HCl. 4. 4. Catalytic groups have pK values of about 8.5 and 9.7 for both membrane-bound and solubilized enzyme. © 1987.
dc.languageeng
dc.relationComparative Biochemistry and Physiology -- Part B: Biochemistry and
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectalkaline phosphatase
dc.subjectdetergent
dc.subjectmacrogol derivative
dc.subjectoctoxinol
dc.subjectanimal
dc.subjectbiosynthesis
dc.subjectbone matrix
dc.subjectcartilage
dc.subjectenzyme induction
dc.subjectenzyme specificity
dc.subjectenzymology
dc.subjectisolation and purification
dc.subjectkinetics
dc.subjectmetabolism
dc.subjectmolecular weight
dc.subjectphysiology
dc.subjectrat
dc.subjectsolubility
dc.subjectAlkaline Phosphatase
dc.subjectAnimal
dc.subjectBone Matrix
dc.subjectCartilage
dc.subjectDetergents
dc.subjectEnzyme Induction
dc.subjectKinetics
dc.subjectMolecular Weight
dc.subjectOctoxynol
dc.subjectPolyethylene Glycols
dc.subjectRats
dc.subjectSolubility
dc.subjectSubstrate Specificity
dc.subjectSupport, Non-U.S. Gov't
dc.titleTriton X-100 solubilized bone matrix-induced alkaline phosphatase
dc.typeOtro


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