Individual Human Cytotoxic T Lymphocytes Exhibit Intraclonal Heterogeneity during Sustained Killing

dc.creatorVasconcelos, Zilton Farias Meira de
dc.creatorMüller, Sabina
dc.creatorGuipouy, Delphine
dc.creatorYu, Wong
dc.creatorChristophe, Claire
dc.creatorGadat, Sébastien
dc.creatorValitutti, Salvatore
dc.creatorDupré, Loïc
dc.date2020-09-30T18:53:37Z
dc.date2020-09-30T18:53:37Z
dc.date2015
dc.date.accessioned2023-09-26T20:28:21Z
dc.date.available2023-09-26T20:28:21Z
dc.identifierVASCONCELOS, Zilton Farias Meira de et al. Individual Human Cytotoxic T Lymphocytes Exhibit Intraclonal Heterogeneity during Sustained Killing. Cell Reports, v. 11, n. 9, p. 1474-1485, Jun 2015.
dc.identifier2211-1247
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/43752
dc.identifier10.1016/j.celrep.2015.05.002
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8857663
dc.descriptionSupplemental Information includes Supplemental Statistical Methods, four fig-ures, and six movies and can be found with this article online athttp:10.1016/j.celrep.2015.05.002.//dx.doi.org/
dc.description1K08DK093709-01/DK/NIDDK NIH HHS/United States
dc.descriptionThe killing of antigen-bearing cells by clonal populations of cytotoxic T lymphocytes (CTLs) is thought to be a rapid phenomenon executed uniformly by individual CTLs. We combined bulk and single-CTL killing assays over a prolonged time period to provide the killing statistics of clonal human CTLs against an excess of target cells. Our data reveal efficiency in sustained killing at the population level, which relied on a highly heterogeneous multiple killing performance at the individual level. Although intraclonal functional heterogeneity was a stable trait in clonal populations, it was reset in the progeny of individual CTLs. In-depth mathematical analysis of individual CTL killing data revealed a substantial proportion of high-rate killer CTLs with burst killing activity. Importantly, such activity was delayed and required activation with strong antigenic stimulation. Our study implies that functional heterogeneity allows CTL populations to calibrate prolonged cytotoxic activity to the size of target cell populations.
dc.formatapplication/pdf
dc.languageeng
dc.publisherCell Press
dc.rightsopen access
dc.subjectCytotoxicity, Immunologic / immunology
dc.subjectFlow Cytometry
dc.subjectHumans
dc.subjectMicroscopy, Confocal
dc.subjectModels, Theoretical
dc.subjectT-Lymphocytes, Cytotoxic / immunology
dc.titleIndividual Human Cytotoxic T Lymphocytes Exhibit Intraclonal Heterogeneity during Sustained Killing
dc.typeArticle


Este ítem pertenece a la siguiente institución