Isolation of a fragment homologous to the rp49 constitutive gene of Drosophila in the Neotropical malaria vector Anopheles aquasalis (Diptera: Culicidae)

dc.creatorGentile, C.
dc.creatorLima, J. B. P.
dc.creatorPeixoto, A. A.
dc.date2020-11-20T18:49:50Z
dc.date2020-11-20T18:49:50Z
dc.date2005
dc.date.accessioned2023-09-26T20:28:07Z
dc.date.available2023-09-26T20:28:07Z
dc.identifierGENTILE, C.; LIMA, J. B. P.; PEIXOTO, A. A. Isolation of a fragment homologous to the rp49 constitutive gene of Drosophila in the Neotropical malaria vector Anopheles aquasalis (Diptera: Culicidae). Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 100, n. 6, p. 545-547, Oct. 2005.
dc.identifier0074-0276
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/44559
dc.identifier10.1590/S0074-02762005000600008
dc.identifier1678-8060
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8857572
dc.descriptionThe constitutive ribosomal gene rp49 is frequently used as an endogenous control in Drosophila gene expression experiments. Using the degenerate primer PCR technique we have cloned a fragment homologous to this gene in Anopheles aquasalis Curry, a Neotropical vector of malaria. In addition, based on this first sequence, a new primer was designed, which allowed the isolation of fragments of rp49 in two other species, Aedes aegypti (Linnaeus) and Culex quinquefasciatus Say, suggesting that it could be used to clone fragments of this gene in a number of other mosquito species. Primers were also designed to specifically amplify rp49 cDNA fragments in An. aquasalis and Ae. aegypti, showing that rp49 could be used as a good constitutive control in gene expression studies of these and other vectorially important mosquito species.
dc.formatapplication/pdf
dc.languageeng
dc.publisherFundação Oswaldo Cruz. Instituto Oswaldo Cruz.
dc.rightsopen access
dc.subjectAnopheles aquasalis
dc.subjectAedes aegypti
dc.subjectCulex quinquefasciatus
dc.subjectDrosophila constitutive gene
dc.subjectrp49
dc.subjectrpL32
dc.subjectMosquitos
dc.subjectAnopheles aquasalis
dc.subjectAedes aegypti
dc.subjectCulex quinquefasciatus
dc.subjectDrosophila constitutive gene
dc.subjectrp49
dc.subjectrpL32
dc.subjectMosquitoes
dc.titleIsolation of a fragment homologous to the rp49 constitutive gene of Drosophila in the Neotropical malaria vector Anopheles aquasalis (Diptera: Culicidae)
dc.typeArticle


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