Human anti-saliva immune response following experimental exposure to the visceral leishmaniasis vector, Lutzomyia longipalpis.

dc.creatorVinhas, Vera
dc.creatorAndrade, Bruno de Bezerril
dc.creatorPaes, Fábio
dc.creatorBomura, Andréa
dc.creatorClarêncio, Jorge
dc.creatorMiranda, José Carlos
dc.creatorBafica, Andre Luiz Barbosa
dc.creatorBarral, Aldina Maria Prado
dc.creatorBarral Netto, Manoel
dc.date2014-03-17T17:40:44Z
dc.date2014-03-17T17:40:44Z
dc.date2007
dc.date.accessioned2023-09-26T20:27:46Z
dc.date.available2023-09-26T20:27:46Z
dc.identifierVINHAS, V. et al. Human anti-saliva immune response following experimental exposure to the visceral leishmaniasis vector, Lutzomyia longipalpis. European Journal of Immunology, v. 37, p. 3111–3121, 2007.
dc.identifier0014-2980
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/7412
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8857459
dc.descriptionExperiments in animals verified that phlebotomine saliva enhances Leishmania infection, and vaccination with saliva prevents disease. We have shown that individuals from an endemic area of visceral leishmaniasis displayed robust antibody responses to saliva from the vector Lutzomyia longipalpis, which correlated with anti-parasite cellmediated immunity. Here, we explored human anti-saliva responses following exposure to sand flies, using an in vivo bite model in which normal volunteers were exposed four times to 30 laboratory-reared Lu. longipalpis. Following the third exposure, normal volunteers developed diverse dermatological reactions at the site of insect bite. Serum from normal volunteers displayed high levels of anti-salivary gland sonicate IgG1, IgG4 and IgE as well as several salivary gland proteins. Furthermore, following in vitro stimulation with salivary gland sonicate, there was an increased frequency of CD4+CD25+ and CD8+CD25+ T cells as well as IFN-c and IL-10 synthesis. Strikingly, 1 year after the first exposure, PBMC from the volunteers displayed recall IFN-c responses that correlated with a significant reduction in infection rates using a macrophage-lymphocyte autologous culture. Together, these data suggest that human immunization against sand fly saliva is feasible and recall responses are obtained even 1 year after exposure, opening perspectives for vaccination in man.
dc.formatapplication/pdf
dc.languageeng
dc.publisherWILEY-VCH Verlag GmbH & Co.
dc.rightsopen access
dc.subjectProteínas de Insetos/imunologia
dc.subjectInsetos Vetores/imunologia
dc.subjectLeishmaniose Visceral/prevenção & controle
dc.subjectPsychodidae/imunologia
dc.subjectSaliva/imunologia
dc.subjectAdulto
dc.subjectAnimais
dc.subjectWestern Blotting
dc.subjectCitocinas/sangue
dc.subjectCitocinas/imunologia
dc.subjectEnsaio de Imunoadsorção Enzimática
dc.subjectFeminino
dc.subjectCitometria de Fluxo
dc.subjectHumanos
dc.subjectImunoglobulina E/sangue
dc.subjectImunoglobulina G/sangue
dc.subjectMemória Imunológica
dc.subjectLeishmaniose Visceral/transmissão
dc.subjectMasculino
dc.subjectProteínas e Peptídeos Salivares/imunologia
dc.subjectLinfócitos T/imunologia
dc.titleHuman anti-saliva immune response following experimental exposure to the visceral leishmaniasis vector, Lutzomyia longipalpis.
dc.typeArticle


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