Release of cytokines by stimulated peripheral blood mononuclear cells in chronic periodontitis

dc.creatorGonçalves, Thais Oliveira
dc.creatorCosta, Dirceu Joaquim
dc.creatorBrodskyn, Claudia Ida
dc.creatorDuarte, Poliana Mendes
dc.creatorCésar Neto, João Batista
dc.creatorNogueira Filho, Getulio
dc.date2014-09-16T14:04:52Z
dc.date2014-09-16T14:04:52Z
dc.date2010
dc.date.accessioned2023-09-26T20:27:06Z
dc.date.available2023-09-26T20:27:06Z
dc.identifierGONÇALVES, T. O. et al. Release of cytokines by stimulated peripheral blood mononuclear cells in chronic periodontitis. Archives of Oral Biology, v. 55, n. 12, p. 975-980, 2010.
dc.identifier1879-1506
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/8386
dc.identifier10.1016/j.archoralbio.2010.08.002
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8857198
dc.descriptionObjective: The emergence of periodontal medicine increased interest in defining the behaviour of peripheral blood cells in periodontitis subjects in comparison with healthy group. The aim of this study was to evaluate the levels of interleukin (IL)-8, tumour necrosis factora (TNF-a), IL-6 and IL-10 released by Escherichia coli lipopolysaccharide (LPS)-stimulated peripheral blood mononuclear cells (PBMC) obtained from the peripheral blood of chronic periodontitis subjects. Design: PBMC samples were isolated from 19 systemically healthy donors, divided into generalized chronic periodontitis (n = 10) and healthy (n = 9) subjects. Cells were incubated for 24–48 h in 500 mL wells containing RPMI 1640 and stimulated with 1.0 ng/mL of E. coli LPS. Supernatants were used to quantify the amounts of IL-8, TNF-a, IL-6 and IL-10 released using enzyme-linked immunosorbent assay (ELISA). Results: PBMC cells from periodontitis subjects released higher levels of TNF-a and IL-6 than those from healthy subjects (P < 0.05). Conversely, the supernatants of the stimulated PBMC cells obtained from healthy subjects presented higher amounts of IL-8 than those from periodontitis (P < 0.05). No differences were observed in the levels of IL-10 (P > 0.05) between groups. Conclusion: In conclusion, the results of the present study showed that E. coli LPS-stimulated PBMC from subjects with periodontitis present a different pattern of cytokine release when compared to PBMC from healthy subjects. This phenomenon could have implications locally, in periodontitis, as well as in systemic diseases.
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier Ltd
dc.rightsopen access
dc.subjectPeriodontitis
dc.subjectCytokines
dc.subjectELISA
dc.subjectMononuclear cell
dc.subjectPBMC
dc.subjectPeriodontite Crônica/sangue
dc.subjectCitocinas/sangue
dc.subjectLeucócitos Mononucleares/metabolismo
dc.subjectAdulto
dc.subjectIdoso
dc.subjectCélulas Cultivadas
dc.subjectEscherichia coli
dc.subjectFeminino
dc.subjectHemorragia Gengival/sangue
dc.subjectHumanos
dc.subjectMediadores da Inflamação/sangue
dc.subjectInterleucina-10/sangue
dc.subjectLeucócitos Mononucleares/efeitos de drogas
dc.subjectLipopolissacarídeos/farmacologia
dc.subjectMasculino
dc.subjectMeia-Idade
dc.subjectPerda da Inserção Periodontal/sangue
dc.subjectBolsa Periodontal/sangue
dc.subjectHábito de Fumar
dc.subjectFator de Necrose Tumoral alfa/sangue
dc.titleRelease of cytokines by stimulated peripheral blood mononuclear cells in chronic periodontitis
dc.typeArticle


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