Single-tube nested PCR assay with in-house DNA extraction for Mycobacterium tuberculosis detection in blood and urine

dc.creatorLima, Juliana Figueirêdo da Costa
dc.creatorGuedes, Gabriela de Moraes Rêgo
dc.creatorLima, Juliana Falcão de Araújo
dc.creatorLira, Laís Ariane de Siqueira
dc.creatorSantos, Fabiana Cristina Fulco
dc.creatorArruda, Mercia Eliane de
dc.creatorMontenegro, Lílian Maria Lapa
dc.creatorSchindler, Haiana Charifker
dc.date2017-09-13T13:31:01Z
dc.date2017-09-13T13:31:01Z
dc.date2015
dc.date.accessioned2023-09-26T20:26:59Z
dc.date.available2023-09-26T20:26:59Z
dc.identifierLIMA, J. F. DA C. et al. Single-tube nested PCR assay with in-house DNA extraction for Mycobacterium tuberculosis detection in blood and urine. Revista Da Sociedade Brasileira De Medicina Tropical, v. 48, n. 6, p. 731–738, dez. 2015.
dc.identifier1678-9849
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/20999
dc.identifier10.1590/0037-8682-0210-2015
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8857153
dc.descriptionMolecular analyses are auxiliary tools for detecting Koch's bacilli in clinical specimens from patients with suspected tuberculosis (TB). However, there are still no efficient diagnostic tests that combine high sensitivity and specificity and yield rapid results in the detection of TB. This study evaluated single-tube nested polymerase chain reaction (STNPCR) as a molecular diagnostic test with low risk of cross contamination for detecting Mycobacterium tuberculosis in clinical samples.
dc.formatapplication/pdf
dc.languagepor
dc.rightsopen access
dc.subjectDNA Bacteriano
dc.subjectMycobacterium tuberculosis
dc.subjectReação em Cadeia da Polimerase
dc.subjectTuberculose
dc.subjectAdolescent
dc.subjectAdult
dc.subjectAged
dc.subjectDNA, Bacterial
dc.subjectFemale
dc.subjectHumans
dc.subjectMale
dc.subjectMiddle Aged
dc.subjectMycobacterium tuberculosis
dc.subjectgenetics
dc.subjectisolation & purification
dc.subjectPolymerase Chain Reaction
dc.subjectTuberculosis
dc.subjectReproducibility of Results
dc.subjectSensitivity and Specificity
dc.subjectYoung Adult
dc.subjectAdolescente
dc.subjectAdulto
dc.subjectIdoso
dc.subjectDNA Bacteriano
dc.subjectFeminino
dc.subjectHumanos
dc.subjectMasculino
dc.subjectMeia-Idade
dc.subjectMycobacterium tuberculosis
dc.subjectReação em Cadeia da Polimerase
dc.subjectReprodutibilidade dos Testes
dc.subjectSensibilidade e Especificidade
dc.subjectTuberculose
dc.subjectAdulto Jovem
dc.titleSingle-tube nested PCR assay with in-house DNA extraction for Mycobacterium tuberculosis detection in blood and urine
dc.typeArticle


Este ítem pertenece a la siguiente institución