Cell-free antigens of Sporothrix brasiliensis: antigenic diversity and application in an immunoblot assay

dc.creatorAlmeida-Paes, Rodrigo
dc.creatorBailão, Alexandre Melo
dc.creatorPizzini, Cláudia Vera
dc.creatorReis, Rosani Santos
dc.creatorSoares, Célia Maria de Almeida
dc.creatorPeralta, José Mauro
dc.creatorGutierrez-Galhardo, Maria Clara
dc.creatorZancopé-Oliveira, Rosely Maria
dc.date2019-10-22T14:09:32Z
dc.date2019-10-22T14:09:32Z
dc.date2012
dc.date.accessioned2023-09-26T20:26:52Z
dc.date.available2023-09-26T20:26:52Z
dc.identifierALMEIDA-PAES, Rodrigo et al. Cell-free antigens of Sporothrix brasiliensis: antigenic diversity and application in an immunoblot assay. Mycoses, v. 55, p. 467-475, 2012.
dc.identifier0933-7407
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/36636
dc.identifier10.1111/j.1439-0507.2012.02175.x
dc.identifier1439-0507
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8857109
dc.descriptionSporotrichosis is a subcutaneous mycosis diagnosed by isolation of the fungus in culture. Serological tests for help in diagnosis in general do not use purified or recombinant antigens, because there is a paucity of described immunoreactive proteins, especially for the new described Sporothrix species, such as Sporothrix brasiliensis. This study aims to characterise antigens from S. brasiliensis and verify their application in serodiagnosis of sporotrichosis. An immunoblot assay allied with computer-based analysis was used to identify putative antigenic molecules in a cell-free extracts of both morphological phases of this fungus, and to delineate antigenic polymorphism among seven S. brasiliensis isolates and one S. schenckii Brazilian strain. The mycelial and yeast phase of the fungus originated 14 and 23 reactive bands, respectively, which were variable in intensity. An 85 kDa antigen, verified in the yeast phase of the fungus, was observed in all strains used and the immunodominant protein was identified. This protein, however, cross-react with serum samples from patients infected with other pathogens. The results show that the S. brasiliensis cell-free antigen extract is a single and inexpensive source of antigens, and can be applied on the sporotrichosis serodiagnosis.
dc.description2020-10-22
dc.formatapplication/pdf
dc.languageeng
dc.publisherWiley
dc.rightsopen access
dc.subjectAntigen
dc.subjectImmunoblot
dc.subjectSporothrix brasiliensis
dc.subjectSporotrichosis
dc.titleCell-free antigens of Sporothrix brasiliensis: antigenic diversity and application in an immunoblot assay
dc.typeArticle


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