Interleukin 21 receptor affects adipogenesis of human adipose-derived stem/stromal cells

dc.creatorFalavinha, Bruna Cristina
dc.creatorBarisón, María Julia
dc.creatorRebelatto, Carmen Lúcia Kuniyoshi
dc.creatorMarcon, Bruna Hilzendeger
dc.creatorAguiar, Alessandra de Melo
dc.creatorSilva, Evelin Brandão da
dc.creatorStimamiglio, Marco Augusto
dc.creatorShigunov, Patrícia
dc.date2022-03-24T12:26:41Z
dc.date2022-03-24T12:26:41Z
dc.date2022
dc.date.accessioned2023-09-26T20:26:09Z
dc.date.available2023-09-26T20:26:09Z
dc.identifierFALAVINHA, Bruna Cristina et al. Interleukin 21 receptor affects adipogenesis of human adipose-derived stem/stromal cells. Stem Cells International, p. 1–14, 2022.
dc.identifier1687-9678
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/51851
dc.identifier10.1155/2022/4930932
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8856844
dc.descriptionDysfunctions in adipose tissue cells are responsible for several obesity-related metabolic diseases. Understanding the process of adipocyte formation is thus fundamental for understanding these diseases. The adipocyte differentiation of adipose-derived stem/stromal cells (ADSCs) showed a reduction in the mRNA level of the interleukin 21 receptor (IL21R) during this process. Although the receptor has been associated with metabolic diseases, few studies have examined its function in stem cells. In this study, we used confocal immunofluorescence assays to determine that IL21R colocalizes with mitochondrial protein ATP5B, ALDH4A1, and the nucleus of human ADSCs. We demonstrated that silencing and overexpression of IL21R did not affect the cell proliferation and mitochondrial activity of ADSCs. However, IL21R silencing did reduce ADSC adipogenic capacity. Further studies are needed to understand the mechanism involved between IL21R and the adipogenic differentiation process.
dc.formatapplication/pdf
dc.languagepor
dc.publisherHindawi
dc.rightsopen access
dc.subjectAdipocytes
dc.subjectMesenchymal Stem Cells
dc.subjectReceptors, Interleukin-21
dc.subjectAdipocitos
dc.subjectCélulas Madre Mesenquimatosas
dc.subjectReceptores de Interleucina-21
dc.subjectAdipocytes
dc.subjectCellules souches mésenchymateuses
dc.subjectRécepteurs à l'interleukine-21
dc.subjectAdipócitos
dc.subjectCélulas-Tronco Mesenquimais
dc.subjectReceptores de Interleucina-21
dc.titleInterleukin 21 receptor affects adipogenesis of human adipose-derived stem/stromal cells
dc.typeArticle


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