Superparamagnetic iron oxide nanoparticles as a tool to track mouse neural stem cells in vivo

dc.creatorAzevedo-Pereira, Ricardo Luiz
dc.creatorRangel, Bárbara
dc.creatorTovar-Moll, Fernanda
dc.creatorGasparetto, Emerson Leandro
dc.creatorAttias, Marcia
dc.creatorZaverucha-do-Valle, Camila
dc.creatorJasmin, null
dc.creatorMendez-Otero, Rosalia
dc.date2019-01-29T12:18:11Z
dc.date2019-01-29T12:18:11Z
dc.date2018
dc.date.accessioned2023-09-26T20:25:27Z
dc.date.available2023-09-26T20:25:27Z
dc.identifierAZEVEDO-PEREIRA, Ricardo Luiz et al. Superparamagnetic iron oxide nanoparticles as a tool to track mouse neural stem cells in vivo. Molecular Biology Reports, p. 1-8, 12 Nov. 2018.
dc.identifier0301-4851
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/31335
dc.identifier10.1007/s11033-018-4460-9
dc.identifier1573-4978
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8856585
dc.descriptionCell transplantation offers a promising approach in many neurological disorders. Neural stem (NS) cells are potential candidates for cell therapy. The ability to track the grafted cells in the host tissue will refine this therapy. Superparamagnetic iron oxide nanoparticles (SPION) have been suggested as a feasible method, but there is no consensus about its safety. Here we investigated the feasibility of label NS cells with SPION and track by MRI after transplantation into mouse striatum with SPION cells and its therapeutic effects by grafting the cells into mouse striatum. We demonstrated that SPION-labeled NS cells display normal patterns of cellular processes including proliferation, migration, differentiation and neurosphere formation. Transmission electron microscopy reveals SPION in the cytoplasm of the cells, which was confirmed by microanalysis. Neurons and astrocytes generated from SPION-labeled NS cells were able to carry nanoparticles after 7 days under differentiation. SPION-labeled NS cells transplanted into striatum of mice were detected by magnetic resonance imaging (MRI) and microscopy 51 days later. In agreement with others reports, we demonstrated that NS cells are able to incorporate SPION in vitro without altering the stemness, and can survive and be tracked by MRI after they have been grafted into mice striatum.
dc.formatapplication/pdf
dc.languageeng
dc.publisherSpringer
dc.rightsrestricted access
dc.subjectNeural stem cells
dc.subjectSuperparamagnetic iron oxide nanoparticle
dc.subjectMagnetic resonance imaging
dc.subjectCellular therapy
dc.subjectCell tracking
dc.titleSuperparamagnetic iron oxide nanoparticles as a tool to track mouse neural stem cells in vivo
dc.typeArticle


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