Molecular cloning and structural modelling of gamma-phospholipase A2 inhibitors from Bothrops atrox and Micrurus lemniscatus snakes

dc.creatorPicelli, Carina G.
dc.creatorBorges, Rafael J.
dc.creatorFernandes, Carlos A. H.
dc.creatorMatioli, Fabio M.
dc.creatorFernandes, Carla Freire Celedônio
dc.creatorSobrinho, Juliana C.
dc.creatorHolanda, Rudson Jesus
dc.creatorOzaki, Luiz S.
dc.creatorKayano, Anderson Makoto
dc.creatorCalderon, Leonardo de Azevedo
dc.creatorFontes, Marcos R. M.
dc.creatorStábeli, Rodrigo Guerino
dc.creatorSoares, Andreimar Martins
dc.date2019-06-11T19:58:11Z
dc.date2019-06-11T19:58:11Z
dc.date2017
dc.date.accessioned2023-09-26T20:24:57Z
dc.date.available2023-09-26T20:24:57Z
dc.identifierPICELLI, Carina G. et al. Molecular cloning and structural modelling of gamma-phospholipase A2 inhibitors from Bothrops atrox and Micrurus lemniscatus snakes. International Journal of Biological Macromolecules, v. 103, p. 525-532, 2017.
dc.identifier0141-8130
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/33462
dc.identifier10.1016/j.ijbiomac.2017.05.076
dc.identifier1879-0003
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8856392
dc.descriptionO artigo encontra-se disponível para download no site do Editor.
dc.descriptionPhospholipases A2 inhibitors (PLIs) produced by venomous and non-venomous snakαes play essential role in this resistance. These endogenous inhibitors may be classified by their fold in PLIα, PLIβ and PLIγ. Phospholipases A2 (PLA2s) develop myonecrosis in snake envenomation, a consequence that is not efficiently neutralized by antivenom treatment. This work aimed to identify and characterize two PLIs from Amazonian snake species, Bothrops atrox and Micrurus lemniscatus. Liver tissues RNA of specimens from each species were isolated and amplified by RT-PCR using PCR primers based on known PLIγ gene sequences, followed by cloning and sequencing of amplified fragments. Sequence similarity studies showed elevated identity with inhibitor PLIγ gene sequences from other snake species. Molecular models of translated inhibitors’ gene sequences resemble canonical three finger fold from PLIγ and support the hypothesis that the decapeptide (residues 107–116) may be responsible for PLA2 inhibition. Structural studies and action mechanism of these PLIs may provide necessary information to evaluate their potential as antivenom or as complement of the current ophidian accident treatment.
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsopen access
dc.subjectSnakes
dc.subjectBothrops atrox
dc.subjectMicrurus lemniscatus
dc.subjectInhibitors of phospholipases A2
dc.subjectGamma type phospholipase A2 inhibitors
dc.titleMolecular cloning and structural modelling of gamma-phospholipase A2 inhibitors from Bothrops atrox and Micrurus lemniscatus snakes
dc.typeArticle


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