Endogenously-expressed NH2-terminus of circumsporozoite protein interferes with sporozoite invasion of mosquito salivary glands

dc.creatorKojin, Bianca B.
dc.creatorSilva, André Luis Costa da
dc.creatorMaciel, Ceres
dc.creatorHenriques, Dayane Alves
dc.creatorCarvalho, Danilo O.
dc.creatorMartin, Kelcie
dc.creatorMarinotti, Osvaldo
dc.creatorJames, Anthony A.
dc.creatorBonaldo, Myrna C.
dc.creatorCapurro, Margareth Lara
dc.date2016-07-02T18:45:00Z
dc.date2016-07-07T12:13:20Z
dc.date2016-07-02T18:45:00Z
dc.date2016-07-07T12:13:20Z
dc.date2016
dc.date.accessioned2023-09-26T20:24:52Z
dc.date.available2023-09-26T20:24:52Z
dc.identifierKOJIN, Bianca B. et al. Endogenously-expressed NH2-terminus of circumsporozoite protein interferes with sporozoite invasion of mosquito salivary glands. Malaria Journal, v.15:153, 11p, 2016.
dc.identifier1475-2875
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/14673
dc.identifier10.1186/s12936-016-1207-8
dc.identifier1475-2875
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8856367
dc.descriptionBackground: The circumsporozoite protein is the most abundant polypeptide expressed by sporozoites, the malaria parasite stage capable of infecting humans. Sporozoite invasion of mosquito salivary glands prior to transmission is likely mediated by a receptor/ligand-like interaction of the parasites with the target tissues, and the amino (NH2)terminal portion of CSP is involved in this interaction but not the TSR region on the carboxyl (C)-terminus. Peptides based on the NH2-terminal domain could compete with the parasites for the salivary gland receptors and thus inhibit penetration. Methods: Peptides based on the NH2-terminus and TSR domains of the CSP from avian or human malaria para‑ sites, Plasmodium gallinaceum and Plasmodium falciparum, respectively, were expressed endogenously in mosquito haemolymph using a transient (Sindbis virus-mediated) or stable (piggyBac-mediated transgenesis) system. Results: Transient endogenous expression of partial NH2-terminus peptide from P. falciparum CSP in P. gallinaceum-infected Aedes aegypti resulted in a reduced number of sporozoites in the salivary glands. When a transgenic approach was used to express a partial CSP NH2-terminal domain from P. gallinaceum the number of sporozoites in the salivary glands did not show a difference when compared to controls. However, a significant difference could be observed when mosquitoes with a lower infection were analysed. The same result could not be observed with mos‑ quitoes endogenously expressing peptides based on the TSR domain from either P. gallinaceum or P. falciparum. Conclusion: These results support the conclusion that CSP partial NH2-terminal domain can be endogenously expressed to promote a competition for the receptor used by sporozoites to invade salivary glands, and they could be used to block this interaction and reduce parasite transmission. The same effect cannot be obtained with peptides based on the TSR domain.
dc.formatapplication/pdf
dc.languageeng
dc.publisherBioMed Central
dc.rightsopen access
dc.subjectPlasmodium gallinaceum
dc.subjectPlasmodium falciparum
dc.subjectCircumsporozoite protein
dc.subjectSindbis virus
dc.subjectTransgenic mosquitoes
dc.subjectAedes aegypti
dc.subjectPlasmodium falciparum
dc.subjectAedes aegypti
dc.subjectVírus Sindbis
dc.subjectPlasmodium gallinaceum
dc.titleEndogenously-expressed NH2-terminus of circumsporozoite protein interferes with sporozoite invasion of mosquito salivary glands
dc.typeArticle


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