Protein synthesis studies in skeletal muscle of aging rats. II. In vitro studies with 0.5M potassium chloride washed polyribosomes

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorBurini, Roberto Carlos
dc.creatorPluskal, M. G.
dc.creatorWei, I.
dc.creatorYoung, V. R.
dc.date2014-05-26T21:55:50Z
dc.date2016-10-25T18:11:47Z
dc.date2014-05-26T21:55:50Z
dc.date2016-10-25T18:11:47Z
dc.date1984-01-01
dc.date.accessioned2017-04-06T00:39:59Z
dc.date.available2017-04-06T00:39:59Z
dc.identifierJournals of Gerontology, v. 39, n. 4, p. 392-397, 1984.
dc.identifier0022-1422
dc.identifierhttp://hdl.handle.net/11449/63647
dc.identifierhttp://acervodigital.unesp.br/handle/11449/63647
dc.identifier10.1093/geronj/39.4.392
dc.identifier2-s2.0-0021238595
dc.identifierhttp://dx.doi.org/10.1093/geronj/39.4.392
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/885633
dc.descriptionTo investigate further the age-related reduction in muscle protein synthesis activity found previously using a crude polyribosome/pH 5 system (Pluskal et al., 1984), a 0.5M KCl washing procedure was utilized to remove the nonribosomal factors from polyribosomes isolated from male Sprague-Dawley rats in the following age groups: young (1 to 2 months), mature (12 months), and aged (22 to 24 months). Using a common source of enriched elongation factor fraction from young animals, it was not possible to demonstrate any significant difference (p > .05) in protein synthesis between the 0.5M KCl-washed polyribosomes isolated from the various age groups. Using a cell-free system containing young salt washed polyribosomes stimulated by the addition of 0.5M KCl-wash fractions, however, it was shown that the mature and aged salt-wash fractions were less (p < .05) active than material from young animals. Thus, the observed decline in protein synthesis efficiency during aging may be attributed to a reduced capacity to promote initiation/elongation by the nonribosomal salt wash fractions of muscle polyribosomes.
dc.languageeng
dc.relationJournals of Gerontology
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectage
dc.subjectaging
dc.subjectanimal experiment
dc.subjectcytology
dc.subjectmuscle
dc.subjectnonhuman
dc.subjectpolysome
dc.subjectprotein synthesis
dc.subjectrat
dc.subjectAging
dc.subjectAmino Acids
dc.subjectAnimal
dc.subjectCell-Free System
dc.subjectIn Vitro
dc.subjectMale
dc.subjectPolyribosomes
dc.subjectPotassium Chloride
dc.subjectProteins
dc.subjectRats
dc.subjectRats, Inbred Strains
dc.subjectSupport, Non-U.S. Gov't
dc.subjectSupport, U.S. Gov't, P.H.S.
dc.titleProtein synthesis studies in skeletal muscle of aging rats. II. In vitro studies with 0.5M potassium chloride washed polyribosomes
dc.typeOtro


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