The activity of platelet activating factor-acetyl hydrolase (PAF-AH) in the salivary glands of Rhodnius prolixus

dc.creatorCôrte-Real, Rozana
dc.creatorGomes, Raquel N.
dc.creatorFaria.Neto, Hugo C. Castro
dc.creatorAzambuja, Patricia
dc.creatorGarcia, Eloi S.
dc.date2018-11-27T15:42:49Z
dc.date2018-11-27T15:42:49Z
dc.date2011
dc.date.accessioned2023-09-26T20:24:05Z
dc.date.available2023-09-26T20:24:05Z
dc.identifierCÔRTE-REAL, Rozana; et al. The activity of platelet activating factor-acetyl hydrolase (PAF-AH) in the salivary glands of Rhodnius prolixus. Journal of Insect Physiology. v.57, p.825–829, 2011.
dc.identifier0022-1910
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/30261
dc.identifier10.1016/j.jinsphys.2011.03.016
dc.identifier1879-1611
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8856090
dc.descriptionIn this work, we investigated the activity of the platelet activating factor acetyl hydrolase (PAF-AH) in the salivary gland homogenates and saliva of Rhodnius prolixus. PAF-AH activity in the salivary gland homogenates was lower than in the saliva. Preliminary characterization of the enzyme demonstrated that it hydrolyzed the substrate 2-thio-PAF, was detectable just in 1 pair of salivary gland homogenates in 0.5 ml buffer, and was stable under different conditions. PMSF, TPCK, TLCK, pepstatin A and p-BPB all inhibited the PAF-AH activity. Enzyme specific activity in salivary gland homogenates diminished immediately after feeding of 5th-instar larvae, and increased before feeding by adult insects. 2-Thio-PAF induced platelet-aggregation that was inhibited by previous incubation of the substrate with salivary gland homogenates or saliva. The relevance of PAF-AH for providing Rhodnius with a feeding mechanism for facilitating the sucking of a high volume of blood meal in a short period is discussed.
dc.description2030-01-01
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsrestricted access
dc.subjectRhodnius prolixus
dc.subjectAlimentação de sangue
dc.subjectAgregação de plaquetas
dc.subjectRhodnius prolixus
dc.subjectPAF acetyl hydrolase
dc.subjectPlatelet-aggregation
dc.subjectBlood feeding
dc.titleThe activity of platelet activating factor-acetyl hydrolase (PAF-AH) in the salivary glands of Rhodnius prolixus
dc.typeArticle


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