Construction and characterization of a recombinant yellow fever virus stably expressing Gaussia luciferase

dc.creatorKassar, Telissa C.
dc.creatorMagalhães, Tereza
dc.creatorS., José V. J.
dc.creatorCarvalho, Amanda G. O.
dc.creatorSilva, Andréa N. M. R. da
dc.creatorQueiroz, Sabrina R. A.
dc.creatorBertani, Giovani R.
dc.creatorGil, Laura H. V. G.
dc.date2018-01-17T14:47:40Z
dc.date2018-01-17T14:47:40Z
dc.date2017
dc.date.accessioned2023-09-26T20:23:48Z
dc.date.available2023-09-26T20:23:48Z
dc.identifierKASSAR, T. C. et al. Construction and characterization of a recombinant yellow fever virus stably expressing Gaussia luciferase. Anais da Academia Brasileira de Ciencias, v. 89, n. 3 Suppl, p. 2119–2130, 2017.
dc.identifier1678-2690
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/23937
dc.identifier10.1590/0001-3765201720160196
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8855977
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq-470453 / 2012-5).
dc.descriptionYellow fever is an arthropod-borne viral disease that still poses high public health concerns, despite the availability of an effective vaccine. The development of recombinant viruses is of utmost importance for several types of studies, such as those aimed to dissect virus-host interactions and to search for novel antiviral strategies. Moreover, recombinant viruses expressing reporter genes may greatly facilitate these studies. Here, we report the construction of a recombinant yellow fever virus (YFV) expressing Gaussia luciferase (GLuc) (YFV-GLuc). We show, through RT-PCR, sequencing and measurement of GLuc activity, that stability of the heterologous gene was maintained after six passages. Furthermore, a direct association between GLuc expression and viral replication was observed (r2=0.9967), indicating that measurement of GLuc activity may be used to assess viral replication in different applications. In addition, we evaluated the use of the recombinant virus in an antiviral assay with recombinant human alfa-2b interferon. A 60% inhibition of GLuc expression was observed in cells infected with YFV-GLuc and incubated with IFN alfa-2b. Previously tested on YFV inhibition by plaque assays indicated a similar fold-decrease in viral replication. These results are valuable as they show the stability of YFV-GLuc and one of several possible applications of this construct.
dc.formatapplication/pdf
dc.languageeng
dc.rightsopen access
dc.subjectGaussia luciferase
dc.subjectrecombinação homóloga em leveduras
dc.subjectgene repórter
dc.subjectvírus da febre amarela
dc.subjectGaussia luciferase
dc.subjecthomologous recombination in yeast
dc.subjectreporter gene
dc.subjectyellow fever virus
dc.subjectfebre amarela
dc.subjectEstudos Transversais
dc.subjectBrasil
dc.subjectepidemiologia
dc.subjectsaúde pública
dc.titleConstruction and characterization of a recombinant yellow fever virus stably expressing Gaussia luciferase
dc.typeArticle


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