T-cell populations and cytokine expression are impaired in thymus and spleen of protein malnourished BALB/c mice infected with leishmania infantum

dc.creatorEscobar, Sergio Cuervo
dc.creatorBarragán, Monica Losada
dc.creatorPérez, Adriana Umaña
dc.creatorPorrozzi, Renato
dc.creatorVahia, Leonardo Saboia
dc.creatorMiranda, Luisa Helena Monteiro de
dc.creatorMorgado, Fernanda Nazaré
dc.creatorMenezes, Rodrigo Caldas
dc.creatorGómez, Myriam Sánchez
dc.creatorCuervo, Patricia
dc.date2015-06-03T17:56:21Z
dc.date2015-06-03T17:56:21Z
dc.date2014
dc.date.accessioned2023-09-26T20:23:35Z
dc.date.available2023-09-26T20:23:35Z
dc.identifierESCOBAR, Sergio Cuervo et al. T-cell populations and cytokine expression are impaired in thymus and spleen of protein malnourished BALB/c mice infected with leishmania infantum. Plos One, v.9, n.12, p.e114584, 2014.
dc.identifier1932-6203
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/10646
dc.identifier10.1371/journal.pone.0114584
dc.identifier1932-6203
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8855890
dc.descriptionCNPq, Departamento Administrativo de Ciencia, Tecnología e Innovación, FIOCRUZ, National University of Colombia Research Council
dc.descriptionVisceral leishmaniasis (VL) is a parasitic infectious disease that causes significant morbidity and mortality in the tropical and subtropical regions of the world. Although infections with visceralizing Leishmania may be asymptomatic, factors such as undernutrition increase the likelihood of progressing to clinical disease. Protein malnutrition, the most deleterious cause of malnutrition in developing countries, has been considered as a primary risk factor for the development of clinical VL. However, data regarding the immunological basis of this association are scarce. With the aim to analyze the effects of protein malnutrition on Leishmania infantum infection, we used BALB/c mice subjected to control or low protein isocaloric diets. Each animal group was divided into two subgroups and one was infected with L. infantum resulting in four study groups: animals fed 14% protein diet (CP), animals fed 4% protein diet (LP), animals fed 14% protein diet and infected (CPi), and animals fed 4% protein diet and infected (LPi).The susceptibility to L. infantum infection and immune responses were assessed in terms of body and lymphoid organ weight, parasite load, lymphocyte subpopulations, and cytokine expression. LPi mice had a significant reduction of body and lymphoid organ weight and exhibited a severe decrease of lymphoid follicles in the spleen. Moreover, LPi animals showed a significant decrease in CD4+CD8+ Tcells in the thymus, whereas there was an increase of CD4+ and CD8+ T cells percentages in the spleen. Notably, the cytokine mRNA levels in the thymus and spleen of protein malnourished-infected animals were altered compared to the CP mice. Protein malnutrition results in a drastic dysregulation of T cells and cytokine expression in the thymus and spleen of L. infantum-infected BALB/c mice, which may lead to defective regulation of the thymocyte population and an impaired splenic immune response, accelerating the events of a normal course of infection.
dc.formatapplication/pdf
dc.languageeng
dc.publisherPublic Library of Science
dc.rightsopen access
dc.subjectLeishmania infantum
dc.subjectLeishmaniasis, Visceral
dc.subjectAntigens, CD4
dc.subjectAntigens, CD8
dc.subjectLeishmania infantum
dc.subjectLeishmaniose Visceral
dc.subjectAntígenos CD4
dc.subjectAntígenos CD8
dc.titleT-cell populations and cytokine expression are impaired in thymus and spleen of protein malnourished BALB/c mice infected with leishmania infantum
dc.typeArticle


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