Proteomic Analysis of the Secretions of Pseudallescheria boydii, a Human Fungal Pathogen with Unknown Genome

dc.creatorSilva, Bianca Alcântara da
dc.creatorSodré, Cátia Lacerda
dc.creatorGonçalves, Ana Luiza Souza
dc.creatorAor, Ana Carolina
dc.creatorKneipp, Lucimar Ferreira
dc.creatorFonseca, Beatriz Bastos
dc.creatorRozental, Sonia
dc.creatorRomanos, Maria Teresa Villela
dc.creatorSola-Penna, Mauro
dc.creatorPerales, Jonas
dc.creatorKalume, Dário Eluan
dc.creatorSantos, André Luis Souza dos
dc.date2018-04-10T11:32:45Z
dc.date2018-04-10T11:32:45Z
dc.date2012
dc.date.accessioned2023-09-26T20:22:58Z
dc.date.available2023-09-26T20:22:58Z
dc.identifierSILVA, Bianca Alcântara da ; et al. Proteomic Analysis of the Secretions of Pseudallescheria boydii, a Human Fungal Pathogen with Unknown Genome. Journal of Proteome Research, v.11, p.172-188, 2012.
dc.identifier1535-3893
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/25703
dc.identifier1535-3907
dc.identifier10.1021/pr200875x
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8855651
dc.descriptionPseudallescheria boydii is a filamentous fungus that causes a wide array of infections that can affect practically all the organs of the human body. The treatment of pseudallescheriosis is difficult since P. boydii exhibits intrinsic resistance to the majority of antifungal drugs used in the clinic and the virulence attributes expressed by this fungus are unknown. The study of the secretion of molecules is an important approach for understanding the pathogenicity of fungi. With this task in mind, we have shown that mycelial cells of P. boydii were able to actively secrete proteins into the extracellular environment; some of them were recognized by antibodies present in the serum of a patient with pseudallescheriosis. Additionally, molecules secreted by P. boydii induced in vitro irreversible damage in pulmonary epithelial cells. Subsequently, two-dimensional gel electrophoresis combined with mass spectrometry was carried out in order to start the construction of a map of secreted proteins from P. boydii mycelial cells. The two-dimensional map showed that most of the proteins (around 100 spots) were focused at pH ranging from 4 to 7 withmolecular masses ranging from 14 to >117 kDa. Fifty spots were randomly selected, of which 30 (60%) were consistently identified, while 20 (40%) spots generated peptides that showed no resemblance to any known protein from other fungi and/or MS with low quality. Notably, we identified proteins involved in metabolic pathways (energy/carbohydrate, nucleotide, and fatty acid), cell wall remodeling, RNA processing, signaling, protein degradation/nutrition, translation machinery, drug elimination and/or detoxification, protection against environmental stress, cytoskeleton/movement proteins, and immunogenic molecules. Since the genome of this fungus is not sequenced, we performed enzymatic and immunodetection assays in order to corroborate the presence of some released proteins. The identification of proteins actively secreted by P. boydii provides important new information for understanding immune modulation and provides important new perspectives on the biology of this intriguing fungus.
dc.formatapplication/pdf
dc.languageeng
dc.publisherAmerican Chemical Society
dc.rightsrestricted access
dc.subjectPseudallescheria
dc.subjectproteínas secretadas
dc.subjectatributos de virulência
dc.subjectPseudallescheria boydii
dc.subjectsecreted proteins
dc.subjectsecretome
dc.subjectvirulence attributes
dc.titleProteomic Analysis of the Secretions of Pseudallescheria boydii, a Human Fungal Pathogen with Unknown Genome
dc.typeArticle


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