Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of the mitochondrial tryparedoxin peroxidase from Leishmania braziliensis

dc.creatorMorais, Mariana Abrahão Bueno de
dc.creatorSouza, Tatiana de Arruda Campos Brasil de
dc.creatorMurakami, Mario Tyago
dc.date2016-09-02T17:57:41Z
dc.date2016-09-02T17:57:41Z
dc.date2013
dc.date.accessioned2023-09-26T20:22:53Z
dc.date.available2023-09-26T20:22:53Z
dc.identifierMORAIS, Mariana Abrahão Bueno de et al. Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of the mitochondrial tryparedoxin peroxidase from Leishmania braziliensis. Acta Crystallogr. Sect F. Struct. Biol. Cryst. Commun, n. 69, p. 408-411.
dc.identifier1744-3091
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/15600
dc.identifier10.1107/S1744309113003989
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8855613
dc.descriptionThis research was supported by grants from Fundação de Amparo a Pesquisa do Estado de São Paulo (FAPESP), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES).
dc.descriptionTryparedoxin peroxidase (TXNPx) is an essential constituent of the main enzymatic scavenger system for reactive oxygen species (ROS) in trypanosomatids. Genetic studies have demonstrated the importance of this system for the development and virulence of these parasites, representing a potential target for the discovery of new trypanocidal drugs. In this work, the mitochondrial TXNPx from Leishmania braziliensis was cloned, overexpressed, purified and crystallized. The crystals diffracted to 3.3 Å resolution and belonged to space group P4(2)2(1)2, with unit-cell parameters a = b = 131.8, c = 44.4 Å. These studies will contribute to a better understanding of the molecular mechanisms involved in ROS detoxification by trypanosomatids.
dc.formatapplication/pdf
dc.languagepor
dc.rightsopen access
dc.subjectLeishmania braziliensis
dc.subjecttryparedoxin peroxidase
dc.subjectCrystallization
dc.subjectMacromolecular Substances
dc.subjectCrystallography
dc.subjectCristalização
dc.subjectSubstâncias Macromoleculares
dc.subjectCristalografia
dc.titleCloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of the mitochondrial tryparedoxin peroxidase from Leishmania braziliensis
dc.typeArticle


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