Study on the interaction of three classical drugs used in psychiatry in albumin through spectrofluorimetric modeling

dc.creatorCoura, Carla Patrícia de Morais
dc.creatorFragoso, Viviane Muniz da Silva
dc.creatorValdez, Ethel Celene Narvaez
dc.creatorPaulino, Erica Tex
dc.creatorSilva, Dilson
dc.creatorCortez, Célia Martins
dc.date2021-08-25T21:42:03Z
dc.date2021-08-25T21:42:03Z
dc.date2021
dc.date.accessioned2023-09-26T20:21:28Z
dc.date.available2023-09-26T20:21:28Z
dc.identifierCOURA, Carla Patrícia de Morais et al. Study on the interaction of three classical drugs used in psychiatry in albumin through spectrofluorimetric modeling. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, v. 255, 119638, 8 p, 2021.
dc.identifier1386-1425
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/48752
dc.identifier10.1016/j.saa.2021.119638
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8855031
dc.descriptionComparative study of haloperidol (HPD), biperiden (BPD) and clonazepam (CNZ) interactions with human and bovine serum albumin was performed based on fluorescence quenching analysis. We used mathe matical modeling comparing spectrofluorimetric data to obtain information on the possibility of compe tition among three drugs by sites binding. Results showed that the three drugs studied have high affinity for albumin and suggest the existence of two site classes in HSA for HPD and only one class for BPD and CNZ, in the range of concentrations tested for each drug. Among them, only HPD forms complex with HSA. Comparing normalized quenching plots suggested that the primary sites in HSA and BSA for HPD and CNZ are located at subdomain IB, whereas BPD would bind in the subdomain IIA. Considering the competition for binding sites in HSA, titrations of HPD-HSA complex by BPD and CNZ, as well as the titra tion of HSA solution containing CNZ titrated by BPD, show that although the three drugs do not compete with each other for binding sites, their interaction with HSA can cause conformational change in the pro tein, and to increase or decrease the accessibility to binding sites for other drug. This may mean alteration in the free plasma drug concentrations.
dc.formatapplication/pdf
dc.languageeng
dc.publisherScience Direct
dc.rightsopen access
dc.subjectHaloperidol
dc.subjectAlbuminas de soro
dc.subjectEspectrofluorimetria
dc.subjectSítios de Ligação
dc.subjectClonazepam
dc.subjectBiperideno
dc.subjectHaloperidol
dc.subjectBiperiden
dc.subjectClonazepam
dc.subjectSerum albumins
dc.subjectBinding sites
dc.subjectSpectrofluorimetry
dc.subjectBiperideno
dc.titleStudy on the interaction of three classical drugs used in psychiatry in albumin through spectrofluorimetric modeling
dc.typeArticle


Este ítem pertenece a la siguiente institución