Vaccination with a Leishmania infantum HSP70-II null mutant confers long-term protective immunity against Leishmania major infection in two mice models

dc.creatorSolana, José Carlos
dc.creatorRamírez, Laura
dc.creatorCorvo, Laura
dc.creatorOliveira, Camila Indiani de
dc.creatorBarral Netto, Manoel
dc.creatorRequena, José María
dc.creatorIborra, Salvador
dc.creatorSoto, Manuel
dc.date2018-04-05T18:56:36Z
dc.date2018-04-05T18:56:36Z
dc.date2017
dc.date.accessioned2023-09-26T20:21:15Z
dc.date.available2023-09-26T20:21:15Z
dc.identifierSOLANA, J. C. et al. Vaccination with a Leishmania infantum HSP70-II null mutant confers long-term protective immunity against Leishmania major infection in two mice models. PLoS Neglected Tropical Diseases, v. 11, n. 5, p. e0005644, 2017.
dc.identifier1935-2727
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/25650
dc.identifier10.1371/journal.pntd.0005644
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8854951
dc.descriptionMinisterio de Ciencia e Innovacion FISPI14/00366 (FEDER FUNDING)
dc.descriptionThe immunization with genetically attenuated Leishmania cell lines has been associated to the induction of memory and effector T cell responses against Leishmania able to control subsequent challenges. A Leishmania infantum null mutant for the HSP70-II genes has been described, possessing a non-virulent phenotype. Methodology/Principal findings The L. infantum attenuated parasites (LiΔHSP70-II) were inoculated in BALB/c (intravenously and subcutaneously) and C57BL/6 (subcutaneously) mice. An asymptomatic infection was generated and parasites diminished progressively to become undetectable in most of the analyzed organs. However, inoculation resulted in the long-term induction of parasite specific IFN-γ responses able to control the disease caused by a challenge of L. major infective promastigotes. BALB/c susceptible mice showed very low lesion development and a drastic decrease in parasite burdens in the lymph nodes draining the site of infection and internal organs. C57BL/6 mice did not show clinical manifestation of disease, correlated to the rapid migration of Leishmania specific IFN-γ producing T cells to the site of infection. Conclusion/Significance Inoculation of the LiΔHSP70-II attenuated line activates mammalian immune system for inducing moderate pro-inflammatory responses. These responses are able to confer longterm protection in mice against the infection of L. major virulent parasites.
dc.formatapplication/pdf
dc.languageeng
dc.publisherPublic Library of Science
dc.rightsopen access
dc.subjectLeishmania infantum
dc.subjectLeishmania major
dc.subjectVacinas Atenuadas
dc.subjectRatos endogâmicos
dc.subjectAnimais
dc.subjectLeismania infantum
dc.subjectLeishmania major
dc.subjectVaccines, Attenuated
dc.subjectMice, Inbred
dc.subjectAnimals
dc.titleVaccination with a Leishmania infantum HSP70-II null mutant confers long-term protective immunity against Leishmania major infection in two mice models
dc.typeArticle


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