Immunoglobulins G, M, and A against Sporothrix schenckii exoantigens in patients with sporotrichosis before and during Treatment with Itraconazole.

dc.creatorPaes, R. A.
dc.creatorPimenta, M. A.
dc.creatorMonteiro, P. C. F.
dc.creatorNosanchuk, J. D.
dc.creatorZancopé-Oliveira, R. M.
dc.date2019-05-28T14:56:38Z
dc.date2019-05-28T14:56:38Z
dc.date2007
dc.date.accessioned2023-09-26T20:21:11Z
dc.date.available2023-09-26T20:21:11Z
dc.identifierALMEIDA-PAES Rodrigo de et al. Immunoglobulins G, M, and A against Sporothrix schenckii Exoantigens in Patients with Sporotrichosis before and during Treatment with Itraconazole. Clinical Vaccine Immunology, v. 14, p. 1149-1157, 2007.
dc.identifier1556-6811
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/33253
dc.identifier10.1128/CVI.00149-07
dc.identifier1556-679X
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8854925
dc.descriptionSporotrichosis is an important subcutaneous mycosis, with an increasing worldwide incidence. However, few data are available regarding the immunological aspects of Sporothrix schenckii infection, particularly the humoral responses to the fungus. In this study we measured immunoglobulin G (IgG), IgM, and IgA in sera from 41 patients with sporotrichosis before antifungal treatment and from another 35 patients with sporotrichosis during itraconazole treatment by using a recently described S. schenckii exoantigen enzyme-linked immunosorbent assay (ELISA). More than 95% of patients had detectable IgA antibodies, and more than 85% had IgM and IgG antibodies before treatment. The number of patients with IgG antibodies increased to 91% during treatment. Conversely, significantly fewer samples from treated patients were positive for IgM (71%) and IgA (89%). Overall, 78% of patients had detectable levels of all isotypes tested at diagnosis, and this percentage dropped to 62.9% in patients receiving itraconazole. Testing of all three isotypes improved the sensitivity; at least two isotypes were detected in 93% of patients before and 89% after treatment. The reactivity of 94 sera from patients with other diseases and healthy individuals was also tested. Cross-reactivity occurred in 33% of the heterologous sera. Most of them were positive only in one isotype, 8.5% were positive for at least two isotypes, and only one serum (1.1%) was positive for the three isotypes. Antibodies produced during S. schenckii infection are diverse, and we demonstrate that an exoantigen ELISA for the detection of combinations of IgA, IgG, and IgM antibodies is a highly sensitive and specific diagnostic assay for sporotrichosis.
dc.description2019-11-28
dc.formatapplication/pdf
dc.languageeng
dc.publisherAmerican Society for Microbiology
dc.rightsopen access
dc.subjectSporotrichosis
dc.subjectSporothrix schenckii
dc.subjectImmunoglobulin G
dc.subjectImmunoglobulin M
dc.subjectImmunoglobulin A
dc.subjectItraconazole
dc.titleImmunoglobulins G, M, and A against Sporothrix schenckii exoantigens in patients with sporotrichosis before and during Treatment with Itraconazole.
dc.typeArticle


Este ítem pertenece a la siguiente institución