Methicillin-resistant Staphylococcus aureus in Human Milk

dc.creatorNovak, Franz Reis
dc.creatorAlmeida, João Aprigio Guerra de
dc.creatorWarnken, Márcia. Barbosa.
dc.creatorFerreira-Carvalho, B. T.
dc.creatorHagler, A. N.
dc.date2010-08-23T16:00:19Z
dc.date2010-08-23T16:00:19Z
dc.date2000
dc.date.accessioned2023-09-26T20:21:10Z
dc.date.available2023-09-26T20:21:10Z
dc.identifierNOVAK, F. R. et al. Methicillin-resistant Staphylococcus aureus in Human Milk. Mem. Inst. Oswaldo Cruz, Rio de Janeiro, v. 95, n. 1, p. 29-33, jan./fev. 2000.
dc.identifier0074-0276
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/248
dc.identifier10.1590/S0074-02762000000100003
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8854924
dc.descriptionWe collected and analyzed 500 samples of human milk, from five Brazilian cities (100 from each) todetect methicillin-resistant strains of Staphylococcus aureus (MRSA) producing enterotoxins. We found57 strains of MRSA, and the mecA gene, responsible for resistance, was detected in all of them using aspecific molecular probe. We examined 40 strains for the presence of four enterotoxins, after selecting asubset that included all strains from each region, except for the largest sample, from which 10 wererandomly selected. Among these two presented enterotoxin B, and growth in human colostrum andtrypicase soy broth. After 5 h of incubation at 37°C, population sizes were already higher than 9.4 x 105UFC/ml and enterotoxin was released into culture medium and colostrum. Our results stress the importanceof hygiene, sanitary measures, and appropriate preservation conditions to avoid the proliferationof S. aureus in human milk.
dc.formatapplication/pdf
dc.languageeng
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dc.rightsopen access
dc.subjectHuman Milk
dc.subjectMethicillin-resistant
dc.subjectStaphylococcus aureus
dc.subjectEnterotoxin
dc.subjectBrazil
dc.subjectResistência a Meticilina
dc.subjectStaphylococcus aureus - efeitos de drogas
dc.subjectLeite Humano - microbiologia
dc.subjectEnterotoxinas - biossíntese
dc.subjectColostro
dc.subjectHibridização Genética
dc.subjectStaphylococcus aureus - genética
dc.titleMethicillin-resistant Staphylococcus aureus in Human Milk
dc.typeArticle


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