Ultrastructural analysis of microfilariae of Wuchereria bancrofti obtained from persistent carriers after repeated courses of diethylcarbamazine

dc.creatorAlves, L. C.
dc.creatorCavalcanti, M. G. S.
dc.creatorAraújo, H. R. C.
dc.creatorSilva, G. M.
dc.creatorVeras, D. L.
dc.creatorPaiva, M. H. S.
dc.creatorBrayner, F. A.
dc.date2018-07-10T14:27:17Z
dc.date2018-07-10T14:27:17Z
dc.date2009
dc.date.accessioned2023-09-26T20:20:56Z
dc.date.available2023-09-26T20:20:56Z
dc.identifierALVES, L. C. et al. Ultrastructural analysis of microfilariae of Wuchereria bancrofti obtained from persistent carriers after repeated courses of diethylcarbamazine. Micron, v. 40, n. 5–6, p. 659–664, jul. 2009.
dc.identifier1878-4291
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/27400
dc.identifier10.1016/j.micron.2009.01.004
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8854833
dc.descriptionEste trabalho foi financiado pela Fundação Oswaldo Cruz (FIOCRUZ), Conselho Nacional de Ciência e Tecnologia (CNPq) e pela Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco (FACEPE - Proc. Nº. 006/2003).
dc.descriptionSingle dose of diethylcarbamazine (DEC) used in control programs is effective in breaking the transmission of filariasis. In order to investigate the effect of aggressive therapy on Wuchereria bancrofti (Wb) microfilariae, DEC was given to 29 patients who were positive for the circulating filarial antigen (CFA) assay but did not have clinical manifestations of filariasis, at 6 mg/kg/day for 12 days and again six months later using the same dosing regimen. For each patient, microfilarial density and serum CFA were followed up for two years. Ultrastructural analyses on Wb microfilariae obtained after repeated treatment with DEC were also performed. Microfilaremia and antigenemia decreased significantly after 12 months but returned to the initial levels after 24 months. This could indicate, as shown by other authors, that aggressive repeated therapy with DEC alone is ineffective in eradicating adult W. bancrofti, particularly in infected but asymptomatic individuals. The objective of the present study was to analyze the microfilaremic and antigenemic behavior and ultrastructural changes caused by different DEC concentrations in vitro in Wb microfilariae obtained from individuals who were sensitive and refractory to treatment. After in vitro treatment of the microfilariae using 5 and 10 microg/ml of DEC for 1h, ultrastructural analysis revealed low levels of cell damage compared with embryos obtained from individuals from a different area who had never received DEC treatment before. The results obtained suggest that microfilariae from patients who receive repeated aggressive therapy are less sensitive to DEC in vitro.
dc.description2050-01-01
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsrestricted access
dc.subjectMicrofilariae de W. bancrofti
dc.subjectTerapia DEC agressiva
dc.subjectAntigenemia
dc.subjectAnálise ultraestrutural
dc.subjectMicrofilariae of W. bancrofti
dc.subjectAggressive DEC therapy
dc.subjectAntigenemia
dc.subjectUltrastructural analysis
dc.subjectAnimais
dc.subjectAntígenos, Helmintos / sangue
dc.subjectDietilcarbamazina / administração & dosagem
dc.subjectDietilcarbamazina / uso terapêutico
dc.subjectFilariose / quimioterapia
dc.subjectFilariose / parasitologia
dc.subjectFilaricidas / administração & dosagem
dc.subjectFilaricidas / uso terapêutico
dc.subjectHumanos
dc.subjectMicroscopia Eletrônica, Transmissão
dc.subjectParasitemia / quimioterapia
dc.subjectResultado do tratamento
dc.subjectWuchereria bancrofti / efeitos de drogas
dc.subjectWuchereria bancrofti / ultraestrutura
dc.titleUltrastructural analysis of microfilariae of Wuchereria bancrofti obtained from persistent carriers after repeated courses of diethylcarbamazine
dc.typeArticle


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