Evaluation of anti-lived and anti-fixed- Leishmania (Viannia) braziliensis promastigotes IgG antibodies detected by flow cytometry for diagnosis and post-therapeutic cure assessment in localized cutaneous leishmaniasis

dc.creatorPereira, Valéria Rêgo Alves
dc.creatorReis, Luiza de Campos
dc.creatorSouza, Marina de Assis
dc.creatorOliveira, Andresa Pereira de
dc.creatorBrito, Maria Edileuza Felinto de
dc.creatorLage, Paula Souza
dc.creatorAndrade, Mariléia Chaves de
dc.creatorRocha, Roberta Dias Rodrigues
dc.creatorMartins Filho, Olindo Assis
dc.date2014-06-17T14:43:21Z
dc.date2014-06-17T14:43:21Z
dc.date2012
dc.date.accessioned2023-09-26T20:20:39Z
dc.date.available2023-09-26T20:20:39Z
dc.identifierPEREIRA, Valéria Rêgo Alves et al. Evaluation of anti-lived and anti-fixed- Leishmania (Viannia) braziliensis promastigotes IgG antibodies detected by flow cytometry for diagnosis and post-therapeutic cure assessment in localized cutaneous leishmaniasis. Diagnostic Microbiology and Infectious Disease. 2012, vol.74, pp. 292-298
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/7826
dc.identifier10.1016/j.diagmicrobio.2012.06.025
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8854720
dc.descriptionState of Pernambuco Research Foundation (FACEPE), by the Brazilian National Research Council (CNPq), and by the Oswaldo Cruz Foundation (FIOCRUZ).
dc.descriptionThis study aims to investigate a flow cytometry performance–based methodology to detect anti-live (FC-ALPA-IgG) and anti-fixed (FC-AFPA-IgG) Leishmania (Viannia) braziliensis promastigote IgG as a means to monitor post-therapeutic cure of patients with localized cutaneous leishmaniasis (LCL). Serum samples from 30 LCL patients infected with L. (V.) braziliensis were assayed, comparing the IgG reactivity before and after specific treatment with pentavalent antimonial. Reactivities were reported as the percentage of positive fluorescent parasites (PPFP), using a PPFP of 60% as a cut-off value. In the serum dilution of 1:1024, the positive percentage of LCL serum sample for FC-ALPA-IgG and FC-AFPA-IgG was 86% and 90%, respectively, before treatment. Analysis of ∆PPFP that represents the difference between PPFP after and before treatment appeared as a new approach to monitor post-therapeutic IgG reactivity in LCL. Our data support the perspective of using FC-ALPA and FC-AFPA as a useful serologic tool for diagnosis and for post-therapeutic follow-up of LCL patients.
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier B.V.
dc.rightsopen access
dc.subjectLocalized cutaneous leishmaniasis
dc.subjectFlow cytometry
dc.subjectFC-ALPA
dc.subjectFC-AFPA
dc.subjectIgG
dc.subjectL. (V.) braziliensis
dc.titleEvaluation of anti-lived and anti-fixed- Leishmania (Viannia) braziliensis promastigotes IgG antibodies detected by flow cytometry for diagnosis and post-therapeutic cure assessment in localized cutaneous leishmaniasis
dc.typeArticle


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