Effect of dexamethasone and testosterone treatment on the regulation of insulin-degrading enzyme and cellular changes in ventral rat prostate after castration

dc.creatorVieira, Juliany S. B. César
dc.creatorSaraiva, Karina L. A.
dc.creatorBarbosa, Maria C. L.
dc.creatorPorto, Regina C. C.
dc.creatorCresto, Juan C.
dc.creatorPeixoto, Christina A.
dc.creatorWanderley, Maria I.
dc.creatorUdrisar, Daniel P.
dc.date2018-12-27T12:59:16Z
dc.date2018-12-27T12:59:16Z
dc.date2011
dc.date.accessioned2023-09-26T20:20:28Z
dc.date.available2023-09-26T20:20:28Z
dc.identifierVIEIRA, Juliany S. B. César et al. Effect of Dexamethasone and Testosterone Treatment on the Regulation of Insulin-Degrading Enzyme and Cellular Changes in Ventral Rat Prostate after Castration. International Journal of Experimental Pathology, v. 92, n. 4, p. 272–280, ago. 2011.
dc.identifier1365-2613
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/30790
dc.identifier10.1111/j.1365-2613.2011.00772.x
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8854651
dc.descriptionInsulin-degrading enzyme (IDE) has been shown to enhance the binding of androgen and glucocorticoid receptors to DNA in the nuclear compartment. Glucocorticoids cause hyperglycaemia, peripheral resistance to insulin and compensatory hyperinsulinaemia. The aim of the present study was to investigate the effect of dexamethasone (D), testosterone (T) and dexamethasone plus testosterone (D + T) on the regulation of IDE and on the remodelling of rat ventral prostate after castration (C). Castration led to a marked reduction in prostate weight (PW). Body weight was significantly decreased in the castrated animals treated with dexamethasone, and the relative PW was 2.6-fold (±0.2) higher in the D group, 2.8-fold (±0.3) higher in the T group and 6.6-fold (±0.6) higher in the D + T group in comparison with the castrated rats. Ultrastructural alterations in the ventral prostate in response to androgen deprivation were restored after testosterone and dexamethasone plus testosterone treatments and partially restored with dexamethasone alone. The nuclear IDE protein level indicated a 4.3-fold (±0.4) increase in castrated rats treated with D + T when compared with castration alone. Whole-cell IDE protein levels increased approximately 1.5-fold (±0.1), 1.5-fold (±0.1) and 2.9-fold (±0.2) in the D, T and D + T groups, respectively, when compared with castration alone. In conclusion, the present study reports that dexamethasone-induced hyperinsulinaemic condition plus exogenous testosterone treatment leads to synergistic effects of insulin and testosterone in the prostatic growth and in the amount of IDE in the nucleus and whole epithelial cell.
dc.description2050-01-01
dc.formatapplication/pdf
dc.languageeng
dc.rightsrestricted access
dc.subjectCastration
dc.subjectGlucocorticoid
dc.subjectInsulin resistance
dc.subjectInsulin-degrading enzyme
dc.subjectProstate
dc.subjectTestosterone
dc.subjectUltrastructure
dc.subjectAndrógenos / farmacologia
dc.subjectAnimais
dc.subjectPeso Corporal / efeitos de drogas
dc.subjectCastração
dc.subjectNúcleo Celular / efeitos de drogas
dc.subjectNúcleo Celular / metabolismo
dc.subjectNúcleo Celular / Patologia
dc.subjectDexametasona / efeitos adversos
dc.subjectDexametasona / farmacologia
dc.subjectGlucocorticóides / efeitos adversos
dc.subjectGlucocorticóides / farmacologia
dc.subjectHiperinsulinismo / induzido quimicamente
dc.subjectHiperinsulinismo / metabolismo
dc.subjectInsulysin / efeitos de drogas
dc.subjectInsulisina / metabolismo
dc.subjectMasculino
dc.subjectModelos Animal
dc.subjectPróstata / efeitos de drogas
dc.subjectPróstata / metabolismo
dc.subjectPróstata / patologia
dc.subjectRatos
dc.subjectRatos Wistar
dc.subjectTestosterona / farmacologia
dc.titleEffect of dexamethasone and testosterone treatment on the regulation of insulin-degrading enzyme and cellular changes in ventral rat prostate after castration
dc.typeArticle


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