Repeatability of the evaluation of systemic microvascular endothelial function using laser doppler perfusion monitoring: clinical and statistical implications

dc.creatorTibiriçá, Eduardo
dc.creatorMatheus, Alessandra S. M.
dc.creatorNunes, Bruno
dc.creatorSperandei, Sandro
dc.creatorGomes, Marilia B.
dc.date2018-11-26T19:19:43Z
dc.date2018-11-26T19:19:43Z
dc.date2011
dc.date.accessioned2023-09-26T20:18:59Z
dc.date.available2023-09-26T20:18:59Z
dc.identifierTIBIRIÇÁ, Eduardo: et al. Repeatability of the evaluation of systemic microvascular endothelial function using laser doppler perfusion monitoring: clinical and statistical implications. Clinicas, v.66, n.4, p.599-605, Jan. 2011.
dc.identifier1807-5932
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/30210
dc.identifier10.1590/S1807-59322011000400013
dc.identifier1980-5322
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8854053
dc.descriptionOBJECTIVE: An awareness of the repeatability of biological measures is required to properly design and calculate sample sizes for longitudinal interventional studies. We investigated the day-to-day repeatability of measures of systemic microvascular reactivity using laser Doppler perfusion monitoring. METHODS: We performed laser Doppler perfusion monitoring in combination with skin iontophoresis using acetylcholine and sodium nitroprusside as well as post-occlusive reactive and thermal hyperemia twice within two weeks. The repeatability was assessed by calculating the within-subject standard deviations, limits of agreement, typical errors and intra-class correlation coefficients between days 1 and 2. The ratio of the within-subject standard deviation to the mean values obtained on days 1 and 2 (within-subject standard deviation/GM) was used to determine the condition with the best repeatability. RESULTS: Twenty-four healthy subjects, aged 24.6¡3.8 years, were recruited. The area under the curve of the vasodilatory response to post-occlusive reactivity showed marked variability (within-subject standard deviation/GM =0.83), while the area under the curve for acetylcholine exhibited less variability (within-subject standard deviation/ GM =0.52) and was comparable to the responses to sodium nitroprusside and thermal treatment (within-subject standard deviations/GM of 0.67 and 0.56, respectively). The area under the blood flow/time curve for vasodilation during acetylcholine administration required the smallest sample sizes, the area under the blood flow/time curve during post-occlusive reactivity required the largest sample sizes, and the area under the blood flow/time curves of vasodilation induced by sodium nitroprusside and thermal treatment required intermediate sizes. CONCLUSIONS: In view of the importance of random error related to the day-to-day repeatability of laser Doppler perfusion monitoring, we propose an original and robust statistical methodology for use in designing prospective clinical studies.
dc.formatapplication/pdf
dc.languageeng
dc.publisherUniversidade de São Paulo. Faculdade de Medicina
dc.rightsopen access
dc.subjectMicrocirculação da Pele
dc.subjectEstudos clínicos
dc.subjectTamanho da amostra
dc.subjectFluxometria por Doppler a Laser
dc.subjectSkin Microcirculation
dc.subjectLaser Doppler Flowmetry
dc.subjectClinical Studies
dc.subjectSample Size
dc.titleRepeatability of the evaluation of systemic microvascular endothelial function using laser doppler perfusion monitoring: clinical and statistical implications
dc.typeArticle


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