Cytopathological effects of Bacillus sphaericus Cry48Aa/Cry49Aa toxin on binary toxin-susceptible and -resistant Culex quinquefasciatus larvae

dc.creatorMelo, Janaina Viana de
dc.creatorJones, Gareth Wyn
dc.creatorBerry, Colin
dc.creatorVasconcelos, Romero Henrique Teixeira
dc.creatorOliveira, Cláudia Maria Fontes de
dc.creatorFurtado, André Freire
dc.creatorPeixoto, Christina Alves
dc.creatorSilva-Filha, Maria Helena Neves Lobo
dc.date2018-08-06T13:09:13Z
dc.date2018-08-06T13:09:13Z
dc.date2009
dc.date.accessioned2023-09-26T20:18:21Z
dc.date.available2023-09-26T20:18:21Z
dc.identifierDE MELO, Janaina Viana et al. Cytopathological Effects of Bacillus Sphaericus Cry48Aa/Cry49Aa Toxin on Binary Toxin-Susceptible and -Resistant Culex Quinquefasciatus Larvae. Applied and Environmental Microbiology, v. 75, n. 14, p. 4782–4789, jul. 2009.
dc.identifier1098-5336
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/27873
dc.identifier10.1128/AEM.00811-09
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8853806
dc.descriptionEste projeto contou com o apoio de doações do Conselho Nacional de Pesquisa (CNPq 475647 / 2004-1 e 471119 / 2006-2) e da Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES).
dc.descriptionThe Cry48Aa/Cry49Aa mosquitocidal two-component toxin was recently characterized from Bacillus sphaericus strain IAB59 and is uniquely composed of a three-domain Cry protein toxin (Cry48Aa) and a binary (Bin) toxin-like protein (Cry49Aa). Its mode of action has not been elucidated, but a remarkable feature of this protein is the high toxicity against species from the Culex complex, besides its capacity to overcome Culex resistance to the Bin toxin, the major insecticidal factor in B. sphaericus-based larvicides. The goal of this work was to investigate the ultrastructural effects of Cry48Aa/Cry49Aa on midgut cells of Bin-toxin-susceptible and -resistant Culex quinquefasciatus larvae. The major cytopathological effects observed after Cry48Aa/Cry49Aa treatment were intense mitochondrial vacuolation, breakdown of endoplasmic reticulum, production of cytoplasmic vacuoles, and microvillus disruption. These effects were similar in Bin-toxin-susceptible and -resistant larvae and demonstrated that Cry48Aa/Cry49Aa toxin interacts with and displays toxic effects on cells lacking receptors for the Bin toxin, while B. sphaericus IAB59-resistant larvae did not show mortality after treatment with Cry48Aa/Cry49Aa toxin. The cytopathological alterations in Bin-toxin-resistant larvae provoked by Cry48Aa/Cry49Aa treatment were similar to those observed when larvae were exposed to a synergistic mixture of Bin/Cry11Aa toxins. Such effects seemed to result from a combined action of Cry-like and Bin-like toxins. The complex effects caused by Cry48Aa/Cry49Aa provide evidence for the potential of these toxins as active ingredients of a new generation of biolarvicides that conjugate insecticidal factors with distinct sites of action, in order to manage mosquito resistance.
dc.formatapplication/pdf
dc.languageeng
dc.rightsopen access
dc.subjectAnimais
dc.subjectBacilo / metabolismo
dc.subjectToxinas bacterianas / farmacologia
dc.subjectCulex / efeitos de drogas
dc.subjectCulex / ultraestrutura
dc.subjectTrato Gastrointestinal / efeitos de drogas
dc.subjectTrato Gastrointestinal / ultraestrutura
dc.subjectInseticidas / farmacologia
dc.subjectLarva / efeitos de drogas
dc.subjectLarva / ultraestrutura
dc.subjectOrganelas / efeitos de drogas
dc.subjectOrganelas / ultraestrutura
dc.subjectAnálise de Sobrevivência
dc.titleCytopathological effects of Bacillus sphaericus Cry48Aa/Cry49Aa toxin on binary toxin-susceptible and -resistant Culex quinquefasciatus larvae
dc.typeArticle


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