Electrophoretic Analysis of Endonuclease-Generated Fragments of k-DNA, of Esterase Isoenzymes, and of Surface Proteins as Aids for Species Identification of Insect Trypanosomatids

dc.creatorCamargo, E. Plessmann
dc.creatorMattei, D. M.
dc.creatorBarbieri, Clara L.
dc.creatorMorel, Carlos Medicis
dc.date2016-07-05T11:06:50Z
dc.date2016-07-08T12:21:54Z
dc.date2016-07-05T11:06:50Z
dc.date2016-07-08T12:21:54Z
dc.date1982
dc.date.accessioned2023-09-26T20:18:15Z
dc.date.available2023-09-26T20:18:15Z
dc.identifierCAMARGO, E. Plessmann; et al. Electrophoretic Analysis of Endonuclease-Generated Fragments of k-DNA, of Esterase Isoenzymes, and of Surface Proteins as Aids for Species Identification of Insect Trypanosomatids. J. Protozool., v.29, n.2, p.251-258, 1982.
dc.identifier0022-3921
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/14765
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8853770
dc.descriptionIn order to verify the applicability of biochemical methods for species identification of Trypanosomatidae, 13 species of monoxenic trypanosomatids plus the heteroxenous Trypanusuma cruzi were comparatively analyzed by three different biochemical methods. Insect trypanosomatids examined were: Crithidia acanthocephali, C. fasciculata (three varieties), C. luciliae luciliae, C. luciliae thermophila, C. deanei, C. oncopelti, Herpetomonas muscarum muscarum, H. megaseliae, H. samuelpessoai, H. mariadeanei, Leptomonas seymouri, L. collosoma, L. samueli. and BIasiocrithidia culicis. Also included in the survey were aposymbiotic strains of C. dranei and C. oncopelti. Methods used were: electrophoretic profiling of endonuclease-generated fragments of k-DNA, esterase isoenzymes profiling, and polyacrylamide-gel electrophoresis (SDS-PAGE) of radioiodinated cell surface proteins. Interspecific but not intraspecific differences were detected by all three methods among the 13 monoxenic species examined. Thus, it is concluded that these methods can be successfully used, in addition to classical criteria, for species identification of insect trypanosomatids.
dc.formatapplication/pdf
dc.languageeng
dc.publisherSociety of Protozoologists
dc.rightsrestricted access
dc.subjectAnálise eletroforética
dc.subjectInsect Trypanosomatids
dc.subjectAids
dc.subjectEndonuclease-Generated Fragments of k-DNA
dc.subjectElectrophoretic Analysis
dc.subjectEsterase Isoenzymes
dc.subjectIsoenzimas
dc.subjectinseto Tripanosomatídeos
dc.subjectAids
dc.subjectEndonuclease
dc.titleElectrophoretic Analysis of Endonuclease-Generated Fragments of k-DNA, of Esterase Isoenzymes, and of Surface Proteins as Aids for Species Identification of Insect Trypanosomatids
dc.typeArticle


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